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DUS2L Recombinant antibody, PBS Only

DUS2L Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

240192C6

Cat no : 83265-8-PBS

Print datasheet

Synonyms

hDUS2, FLJ20399, EC:1.3.1.91, DUS2, Dihydrouridine synthase 2

验证数据展示

  • WB analysis using 83265-8-RR (same clone as 83265-8-PBS)

    WB analysis using 83265-8-RR (same clone as 83265-8-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83265-8-RR (DUS2L antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83265-8-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83265-8-RR (same clone as 83265-8-PBS)

    FC experiment of HeLa using 83265-8-RR (same clone as 83265-8-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUS2L Recombinant antibody (83265-8-RR, Clone:240192C6) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Control Antibody (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83265-8-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83265-8-RR

    Affinity and Kinetic Characterization of 83265-8-RR

    Biolayer interferometry (BLl) kinetic assays of 83265-8-RR against Human DUS2L were performed. The affinity constant is 0.321 nM.

产品信息

83265-8-PBS targets DUS2L in WB, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity human, mouse
Immunogen DUS2L fusion protein Ag23831 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dihydrouridine synthase 2-like, SMM1 homolog (S. cerevisiae)
Synonyms hDUS2, FLJ20399, EC:1.3.1.91, DUS2, Dihydrouridine synthase 2
Observed Molecular Weight 55 kDa
GenBank Accession NumberBC006527
Gene Symbol DUS2L
Gene ID (NCBI) 54920
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9NX74
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dihydrouridine synthase 2(DUS2L), is a cytoplasmic protein that catalyzes the conversion of uridine residues to dihydrouridine in the D-loop of tRNA. transfection of DUS2 cDNA into a human lung carcinoma cell line increased dihydrouridine in tRNA, whereas transfection of DUS2 small interfering RNA (siRNA) reduced tRNA-dihydrouridine, confirming that DUS2 has dihydrouridine synthase activity.