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DUSP6 Recombinant antibody, PBS Only (Capture)

DUSP6 Uni-rAbTM Recombinant Antibody for FC (Intra), Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC (Intra), Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240303A9

Cat no : 83355-3-PBS

Print datasheet

Synonyms

MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6

验证数据展示

  • FC experiment of PC-12 using 83355-3-RR (same clone as 83355-3-PBS)

    FC experiment of PC-12 using 83355-3-RR (same clone as 83355-3-PBS)

    1x10^6 PC-12 cells were intracellularly stained with 0.25 ug Dusp6 Recombinant Antibody (83355-3-RR, Clone:240303A9) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83355-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83355-3-RR (same clone as 83355-3-PBS)

    FC experiment of HeLa using 83355-3-RR (same clone as 83355-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Dusp6 Recombinant Antibody (83355-3-RR, Clone:240303A9) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83355-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00391-2

    Cytometric bead array standard curve of MP00391-2

    Cytometric bead array standard curve of MP00391-2, DUSP6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83355-3-PBS. Detection antibody: 83355-4-PBS. Standard: Ag0705. Range: 5-80ng/mL.

  • Affinity and Kinetic Characterization of 83355-3-PBS

    Affinity and Kinetic Characterization of 83355-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83355-3-PBS against Human DUSP6 was performed. The affinity constant is 41.7 pM.

  • Affinity and Kinetic Characterization of 83355-3-RR

    Affinity and Kinetic Characterization of 83355-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83355-3-RR against Human DUSP6 were performed. The affinity constant is below 1 pM.

产品信息

83355-3-PBS targets DUSP6 as part of a matched antibody pair:

MP00391-2: 83355-3-PBS capture and 83355-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications FC (Intra), Cytometric bead array, Indirect ELISA Application Description
Tested Reactivity human
Immunogen DUSP6 fusion protein Ag0705 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name dual specificity phosphatase 6
Synonyms MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6
Calculated Molecular Weight42 kDa
GenBank Accession NumberBC003562
Gene Symbol DUSP6
Gene ID (NCBI) 1848
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16828
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).