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验证数据展示

  • FC experiment of PC-12 using 83355-3-RR (same clone as 83355-3-PBS)

    FC experiment of PC-12 using 83355-3-RR (same clone as 83355-3-PBS)

    1x10^6 PC-12 cells were intracellularly stained with 0.25 ug Dusp6 Recombinant Antibody (83355-3-RR, Clone:240303A9) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83355-3-PBS in a different storage buffer formulation.

  • FC experiment of HeLa using 83355-3-RR (same clone as 83355-3-PBS)

    FC experiment of HeLa using 83355-3-RR (same clone as 83355-3-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug Dusp6 Recombinant Antibody (83355-3-RR, Clone:240303A9) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 83355-3-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00391-2

    Cytometric bead array standard curve of MP00391-2

    Cytometric bead array standard curve of MP00391-2, DUSP6 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83355-3-PBS. Detection antibody: 83355-4-PBS. Standard: Ag0705. Range: 5-80ng/mL.

  • Affinity and Kinetic Characterization of 83355-3-PBS

    Affinity and Kinetic Characterization of 83355-3-PBS

    Biolayer interferometry (BLl) kinetic assay of 83355-3-PBS against Human DUSP6 was performed. The affinity constant is 41.7 pM.

  • Affinity and Kinetic Characterization of 83355-3-RR

    Affinity and Kinetic Characterization of 83355-3-RR

    Biolayer interferometry (BLl) kinetic assays of 83355-3-RR against Human DUSP6 were performed. The affinity constant is below 1 pM.

DUSP6 Recombinant antibody, PBS Only (Capture)

DUSP6 Uni-rAbTM Recombinant Antibody for FC (Intra), Cytometric bead array, Indirect ELISA
Cat No. 83355-3-PBS

产品说明书

CloneNo. 240303A9

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

FC (Intra), Cytometric bead array, Indirect ELISA

别名

MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6

缓冲液配方:  PBS Only
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy DUSP6 Ready-To-Use IHC Kit. DUSP6 primary antibody included.

产品信息

83355-3-PBS targets DUSP6 as part of a matched antibody pair:

MP00391-2: 83355-3-PBS capture and 83355-4-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 FC (Intra), Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human
免疫原 DUSP6 fusion protein Ag0705 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dual specificity phosphatase 6
别名 MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6
计算分子量42 kDa
GenBank蛋白编号BC003562
基因名称 DUSP6
Gene ID (NCBI) 1848
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ16828
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).