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验证数据展示

  • WB analysis using 83356-5-RR

    WB analysis using 83356-5-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83356-5-RR (DUSP6 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of HeLa using 83356-5-RR

    FC experiment of HeLa using 83356-5-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • FC experiment of PC-12 using 83356-5-RR

    FC experiment of PC-12 using 83356-5-RR

    1x10^6 PC-12 cells were intracellularly stained with 0.25 ug DUSP6 Recombinant antibody (83356-5-RR, Clone:240295C8) (red) or Isotype Controland (blue) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 83356-5-RR

    Affinity and Kinetic Characterization of 83356-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83356-5-RR against Human DUSP6 were performed. The affinity constant is 3.38 nM.

DUSP6 Recombinant antibody

DUSP6 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 83356-5-RR

产品说明书

CloneNo. 240295C8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, ELISA

别名

MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells, A549 cells, HepG2 cells, NIH/3T3 cells, PC-12 cells, mouse heart tissue, rat heart tissue
Positive FC (Intra) detected inHeLa cells, PC-12 cells
Planning an IHC experiment? We recommend our IHCeasy DUSP6 Ready-To-Use IHC Kit. DUSP6 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83356-5-RR targets DUSP6 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 DUSP6 fusion protein Ag23474 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 dual specificity phosphatase 6
别名 MKP 3, Mitogen-activated protein kinase phosphatase 3, MAP kinase phosphatase 3, EC:3.1.3.16, dual specificity phosphatase 6
计算分子量42 kDa
观测分子量42-45 kDa
GenBank蛋白编号BC003562
基因名称 DUSP6
Gene ID (NCBI) 1848
RRIDAB_3671013
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDQ16828
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dual specificity phosphatase 6 (DUSP6) is a member of the family of mitogen-activated protein kinase (MAPK) phosphatases, which dephosphorylate and inactivate MAPK (PMID: 9858808). The MAPK pathway is an important signal transduction system that serves cellular processes, such as proliferation, differentiation, migration, and apoptosis. DUSP6 resides at chromosome location 12q22-23, which is one of the candidate loci for susceptibility to bipolar disorder and which encodes a phosphatase selective for extracellular signal-regulated kinase (ERK) (PMID: 9205128). DUSP6 inactivates extracellular signal-regulated kinase (ERK), and can act in tumor suppressive pathways, whose expression is reduced in several different types of cancer. (PMID: 21680106).

实验方案

Product Specific Protocols
WB protocol for DUSP6 antibody 83356-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols