DUT Polyclonal antibody
DUT Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, IP, ELISA and More (1)
Conjugate
Unconjugated
验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, human liver tissue, human kidney tissue, HEK-293 cells, Ramos cells, A549 cells, HepG2 cells |
| Positive IP detected in | HEK-293 cells |
| Positive IHC detected in | human heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Planning an IHC experiment? We recommend our IHCeasy DUT Ready-To-Use IHC Kit. DUT primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.
推荐稀释比
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
| CoIP | See 1 publications below |
产品信息
13740-1-AP targets DUT in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Applications | WB, IHC, IF/ICC, IP, ELISA Application Description |
| Cited Applications | WB, CoIP |
| Tested Reactivity | human |
| Cited Reactivity | human |
| Immunogen | DUT fusion protein Ag4677 种属同源性预测 |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Full Name | deoxyuridine triphosphatase |
| Synonyms | EC:3.6.1.23, dUTPase, deoxyuridine triphosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial |
| Calculated Molecular Weight | 252 aa, 27 kDa |
| Observed Molecular Weight | 23 kDa |
| GenBank Accession Number | BC033645 |
| Gene Symbol | DUT |
| Gene ID (NCBI) | 1854 |
| RRID | AB_2093170 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P33316 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for DUT antibody 13740-1-AP | Download protocol |
| IHC protocol for DUT antibody 13740-1-AP | Download protocol |
| IF protocol for DUT antibody 13740-1-AP | Download protocol |
| IP protocol for DUT antibody 13740-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cell Metab Malic enzyme 2 connects the Krebs cycle intermediate fumarate to mitochondrial biogenesis. | ||
Cell Chem Biol CETSA interaction proteomics define specific RNA-modification pathways as key components of fluorouracil-based cancer drug cytotoxicity. | ||
Bioconjug Chem ATP-Linked Chimeric Nucleotide as a Specific Luminescence Reporter of Deoxyuridine Triphosphatase. |
