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CoraLite® Plus 488-conjugated DUT Polyclonal antibody

DUT Polyclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-13740

Print datasheet

Synonyms

EC:3.6.1.23, dUTPase, deoxyuridine triphosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial

验证数据展示

  • IF Staining of HeLa using CL488-13740

    IF Staining of HeLa using CL488-13740

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 DUT antibody (CL488-13740) at dilution of 1:200, CL594-Phalloidin (red).

  • FC experiment of U2OS using CL488-13740

    FC experiment of U2OS using CL488-13740

    1x10^6 U2OS cells were intracellularly stained with 0.8 ug Coralite® Plus 488 DUT Polyclonal Antibody (CL488-13740)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

经过测试的应用

Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inU2OS cells
Planning an IHC experiment? We recommend our IHCeasy DUT Ready-To-Use IHC Kit. DUT primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 13740-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-13740 targets DUT in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen DUT fusion protein Ag4677 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name deoxyuridine triphosphatase
Synonyms EC:3.6.1.23, dUTPase, deoxyuridine triphosphatase, Deoxyuridine 5'-triphosphate nucleotidohydrolase, mitochondrial
Calculated Molecular Weight 252 aa, 27 kDa
Observed Molecular Weight 23 kDa
GenBank Accession NumberBC033645
Gene Symbol DUT
Gene ID (NCBI) 1854
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP33316
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DUT antibody CL488-13740Download protocol
Standard Protocols
Click here to view our Standard Protocols