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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated DVL1 Polyclonal antibody

DVL1 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-27384

Print datasheet

Synonyms

Dishevelled 1, DSH homolog 1, DVL, DVL1

验证数据展示

  • IF Staining of MCF-7 using CL488-27384

    IF Staining of MCF-7 using CL488-27384

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite® Plus 488 DVL1 antibody (CL488-27384) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 27384-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-27384 targets DVL1 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse
Immunogen DVL1 fusion protein Ag26055 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name dishevelled, dsh homolog 1 (Drosophila)
Synonyms Dishevelled 1, DSH homolog 1, DVL, DVL1
Calculated Molecular Weight 75 kDa
Observed Molecular Weight75 kDa, 73 kDa
GenBank Accession NumberBC050454
Gene Symbol DVL1
Gene ID (NCBI) 1855
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO14640
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DVL1 is one of three DVL homologous proteins (DVL1-3) widely expressed in embryonic development and in the adult central nervous system. Dishevelled proteins are a necessary component of the Wnt and planar cell polarity developmental signaling pathways. Overexpression of DVL1 has been linked to prostate and breast cancers through the Wnt signaling pathway.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DVL1 antibody CL488-27384Download protocol
Standard Protocols
Click here to view our Standard Protocols