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验证数据展示

  • WB analysis using 68312-1-Ig

    WB analysis using 68312-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68312-1-Ig (DYNLT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • WB analysis of HEK-293 using 68312-1-Ig

    WB analysis of HEK-293 using 68312-1-Ig

    WB result of DYNLT1 antibody (68312-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-DYNLT1 transfected HEK-293 cells.

  • IF Staining of C2C12 using 68312-1-Ig

    IF Staining of C2C12 using 68312-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using DYNLT1 antibody (68312-1-Ig, Clone: 2E3D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • IF Staining of H9C2 using 68312-1-Ig

    IF Staining of H9C2 using 68312-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using DYNLT1 antibody (68312-1-Ig, Clone: 2E3D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  • KD/KO验证

DYNLT1 Monoclonal antibody

DYNLT1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 68312-1-Ig

产品说明书

CloneNo. 2E3D4

宿主/亚型

Mouse / IgG2a

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

2E3D4, CW 1, Dynein light chain Tctex-type 1, Protein CW 1, Protein CW-1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IF/ICC detected inH9C2 cells, C2C12 cells
Planning an IHC experiment? We recommend our IHCeasy DYNLT1 Ready-To-Use IHC Kit. DYNLT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68312-1-Ig targets DYNLT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag33072

Product name: Recombinant human DYNLT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-113 aa of BC029412

Sequence: MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKPFKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAFGLSI

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 dynein, light chain, Tctex-type 1
别名 2E3D4, CW 1, Dynein light chain Tctex-type 1, Protein CW 1, Protein CW-1
计算分子量 113 aa, 12 kDa
观测分子量12 kDa
GenBank蛋白编号BC029412
基因名称 DYNLT1
Gene ID (NCBI) 6993
RRIDAB_2935390
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP63172
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (PMID: 19448628)

实验方案

Product Specific Protocols
IF protocol for DYNLT1 antibody 68312-1-IgDownload protocol
WB protocol for DYNLT1 antibody 68312-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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