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DYNLT1 Monoclonal antibody

DYNLT1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

2E3D4

Cat no : 68312-1-Ig

Print datasheet

Synonyms

CW 1, DYNLT1, Protein CW 1, TCTEL1, tctex 1, TCTEX1

验证数据展示

  • WB analysis using 68312-1-Ig

    WB analysis using 68312-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68312-1-Ig (DYNLT1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IF Staining of C2C12 using 68312-1-Ig

    IF Staining of C2C12 using 68312-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed C2C12 cells using DYNLT1 antibody (68312-1-Ig, Clone: 2E3D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

  • IF Staining of H9C2 using 68312-1-Ig

    IF Staining of H9C2 using 68312-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using DYNLT1 antibody (68312-1-Ig, Clone: 2E3D4 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

经过测试的应用

Positive WB detected inA549 cells, HeLa cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Planning an IHC experiment? We recommend our IHCeasy DYNLT1 Ready-To-Use IHC Kit. DYNLT1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68312-1-Ig targets DYNLT1 in WB, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen DYNLT1 fusion protein Ag33072 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name dynein, light chain, Tctex-type 1
Synonyms CW 1, DYNLT1, Protein CW 1, TCTEL1, tctex 1, TCTEX1
Calculated Molecular Weight 113 aa, 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC029412
Gene Symbol DYNLT1
Gene ID (NCBI) 6993
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63172
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (PMID: 19448628)

实验方案

Product Specific Protocols
WB protocol for DYNLT1 antibody 68312-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols