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CoraLite® Plus 488-conjugated DYNLT1 Monoclonal antibody

DYNLT1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E3D4

Cat no : CL488-68312

Print datasheet

Synonyms

T-complex testis-specific protein 1 homolog, Protein CW-1, Protein CW 1, Dynein light chain Tctex-type 1, CW 1

验证数据展示

  • IF Staining of HepG2 using CL488-68312

    IF Staining of HepG2 using CL488-68312

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 DYNLT1 antibody (CL488-68312, Clone: 2E3D4 ) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DYNLT1 Ready-To-Use IHC Kit. DYNLT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 68312-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-68312 targets DYNLT1 in IF/ICC applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity human, mouse, rat
Immunogen DYNLT1 fusion protein Ag33072 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name dynein, light chain, Tctex-type 1
Synonyms T-complex testis-specific protein 1 homolog, Protein CW-1, Protein CW 1, Dynein light chain Tctex-type 1, CW 1
Calculated Molecular Weight 113 aa, 12 kDa
Observed Molecular Weight12 kDa
GenBank Accession NumberBC029412
Gene Symbol DYNLT1
Gene ID (NCBI) 6993
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP63172
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

DYNLT (or Tctex-1) was originally described as a light chain component of the dynein motor complex. Tctex-1 also has several dynein-independent functions, including roles in G protein signaling activation and neuronal growth. Tctex-1 is selectively enriched in proliferating neural progenitors of both embryonic and adult brains. Genetic knockdown of Tctex-1 in radial precursors promoted neurogenesis, indicating its implication in regulation of cortical neurogenesis. (PMID: 19448628)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 DYNLT1 antibody CL488-68312Download protocol
Standard Protocols
Click here to view our Standard Protocols