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验证数据展示

  • WB analysis of pig cartilage using 66847-1-Ig

    WB analysis of pig cartilage using 66847-1-Ig

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66847-1-Ig (Decorin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 66847-1-Ig

    IF Staining of BxPC-3 using 66847-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Decorin antibody (66847-1-Ig, Clone: 3A4E4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

Decorin Monoclonal antibody

Decorin Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 66847-1-Ig

产品说明书

CloneNo. 3A4E4

宿主/亚型

Mouse / IgG1

种属反应性

human, pig

应用

WB, IF/ICC, ELISA

别名

DCN, PG40, PG S2, DSPG2, CSCD

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inpig cartilage tissue
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy DCN Ready-To-Use IHC Kit. DCN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66847-1-Ig targets Decorin in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, pig
免疫原 Decorin fusion protein Ag6275 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 decorin
别名 DCN, PG40, PG S2, DSPG2, CSCD
计算分子量 359 aa, 40 kDa
观测分子量 40 kDa, 100 kDa
GenBank蛋白编号BC005322
基因名称 Decorin
Gene ID (NCBI) 1634
RRIDAB_2882187
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP07585
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.

实验方案

Product Specific Protocols
WB protocol for Decorin antibody 66847-1-IgDownload protocol
IF protocol for Decorin antibody 66847-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols