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Decorin Monoclonal antibody

Decorin Monoclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

3A4E4

Cat no : 66847-1-Ig

Print datasheet

Synonyms

DCN, PG40, PG S2, DSPG2, CSCD

验证数据展示

  • WB analysis of pig cartilage using 66847-1-Ig

    WB analysis of pig cartilage using 66847-1-Ig

    pig cartilage tissue were subjected to SDS PAGE followed by western blot with 66847-1-Ig (Decorin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of BxPC-3 using 66847-1-Ig

    IF Staining of BxPC-3 using 66847-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using Decorin antibody (66847-1-Ig, Clone: 3A4E4 ) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

经过测试的应用

Positive WB detected inpig cartilage tissue
Positive IF/ICC detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy DCN Ready-To-Use IHC Kit. DCN primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66847-1-Ig targets Decorin in WB, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity human, pig
Immunogen Decorin fusion protein Ag6275 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name decorin
Synonyms DCN, PG40, PG S2, DSPG2, CSCD
Calculated Molecular Weight 359 aa, 40 kDa
Observed Molecular Weight 40 kDa, 100 kDa
GenBank Accession NumberBC005322
Gene Symbol Decorin
Gene ID (NCBI) 1634
RRIDAB_2882187
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07585
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.

实验方案

Product Specific Protocols
WB protocol for Decorin antibody 66847-1-IgDownload protocol
IF protocol for Decorin antibody 66847-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols