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验证数据展示

  • IF Staining of BxPC-3 using CL488-66847

    IF Staining of BxPC-3 using CL488-66847

    Immunofluorescent analysis of (-20°C Ethanol) fixed BxPC-3 cells using CoraLite® Plus 488 Decorin antibody (CL488-66847, Clone: 3A4E4 ) at dilution of 1:200.

  • FC experiment of BxPC-3 using CL488-66847

    FC experiment of BxPC-3 using CL488-66847

    1x10^6 BxPC-3 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated Decorin Monoclonal antibody (CL488-66847, Clone:3A4E4)(red), or 0.4 ug CoraLite® Plus 488-conjugated Mouse IgG1 isotype control Mouse McAb (CL488-66360, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite® Plus 488-conjugated Decorin Monoclonal antibody

Decorin Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-66847

Print datasheet

CloneNo. 3A4E4

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Synonyms

DCN, PG40, PG S2, DSPG2, CSCD

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inBxPC-3 cells
Positive FC (Intra) detected inBxPC-3 cells
Planning an IHC experiment? We recommend our IHCeasy DCN Ready-To-Use IHC Kit. DCN primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66847-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66847 targets Decorin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human
Immunogen Decorin fusion protein Ag6275 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name decorin
Synonyms DCN, PG40, PG S2, DSPG2, CSCD
Calculated Molecular Weight 359 aa, 40 kDa
Observed Molecular Weight 40 kDa, 100 kDa
GenBank Accession NumberBC005322
Gene Symbol Decorin
Gene ID (NCBI) 1634
RRIDAB_3672925
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP07585
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Decorin antibody CL488-66847Download protocol
FC protocol for CL Plus 488 Decorin antibody CL488-66847Download protocol
Standard Protocols
Click here to view our Standard Protocols