验证数据展示
经过测试的应用
Positive IF/ICC detected in | BxPC-3 cells |
Positive FC (Intra) detected in | BxPC-3 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
CL488-66847 targets Decorin in IF/ICC, FC (Intra) applications and shows reactivity with human samples.
经测试应用 | IF/ICC, FC (Intra) Application Description |
经测试反应性 | human |
免疫原 | Decorin fusion protein Ag6275 种属同源性预测 |
宿主/亚型 | Mouse / IgG1 |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | decorin |
别名 | DCN, PG40, PG S2, DSPG2, CSCD |
计算分子量 | 359 aa, 40 kDa |
观测分子量 | 40 kDa, 100 kDa |
GenBank蛋白编号 | BC005322 |
基因名称 | Decorin |
Gene ID (NCBI) | 1634 |
RRID | AB_3672925 |
偶联类型 | CoraLite® Plus 488 Fluorescent Dye |
最大激发/发射波长 | 493 nm / 522 nm |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P07585 |
储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3 |
储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Decorin is a member of the small leucine-rich proteoglycan family of proteins. Decorin interacts with type I collagen fibrils, thereby influencing the kinetics of fibril formation and the distance between adjacent collagen fibrils. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis.
实验方案
Product Specific Protocols | |
---|---|
IF protocol for CL Plus 488 Decorin antibody CL488-66847 | Download protocol |
FC protocol for CL Plus 488 Decorin antibody CL488-66847 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |