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Desmoplakin Polyclonal antibody

Desmoplakin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 25318-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

DSP, DP, DESP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -


经过测试的应用

Positive WB detected inA431 cells, HepG2 cells, mouse heart tissue, BxPC-3 cells, HEK-293 cells
Positive IP detected inBxPC-3 cells
Positive IHC detected inrat heart tissue, human normal colon, human bowen disease, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

25318-1-AP targets Desmoplakin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
文献引用应用WB, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse, rat
免疫原 Desmoplakin fusion protein Ag18065 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 desmoplakin
别名 DSP, DP, DESP
计算分子量 2871 aa, 332 kDa
观测分子量 240-330 kDa
GenBank蛋白编号BC140802
基因名称 DSP
Gene ID (NCBI) 1832
RRIDAB_2880028
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15924
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

实验方案

Product Specific Protocols
WB protocol for Desmoplakin antibody 25318-1-APDownload protocol
IHC protocol for Desmoplakin antibody 25318-1-APDownload protocol
IF protocol for Desmoplakin antibody 25318-1-APDownload protocol
IP protocol for Desmoplakin antibody 25318-1-APDownload protocol
FC protocol for Desmoplakin antibody 25318-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Bone Res

Overexpression of Lrp5 enhanced the anti-breast cancer effects of osteocytes in bone.

Authors - Shengzhi Liu
mouseWB,IF

Theranostics

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.

Authors - Min Zhang
humanWB

Cancer Lett

ETV4 is a theranostic target in clear cell renal cell carcinoma that promotes metastasis by activating the pro-metastatic gene FOSL1 in a PI3K-AKT dependent manner.

Authors - Liang Xu
mouseWB

Cancers (Basel)

Inhibition of the Growth of Breast Cancer-Associated Brain Tumors by the Osteocyte-Derived Conditioned Medium.

Authors - Tomohiko Sano
humanWB

J Cell Mol Med

Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.

Authors - Lin-Jian Wang
  • KD Validated
humanWB

Cell Death Discov

Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway.

Authors - Yanlei Yang
  • KD Validated
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