验证数据展示

WB analysis of HepG2 using 25318-1-AP
WB result of Desmoplakin antibody (25318-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Desmoplakin transfected HepG2 cells.
WB analysis using 25318-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 25318-1-AP (Desmoplakin antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
IF Staining of A431 using 25318-1-AP
Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using Desmoplakin antibody (25318-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), Beta Actin antibody (66009-1-Ig, Clone: 2D4H5, red).
IP experiment of BxPC-3 using 25318-1-AP
IP result of anti-Desmoplakin (IP:25318-1-AP, 4ug; Detection:25318-1-AP 1:10000) with BxPC-3 cells lysate 2080 ug.
IHC staining of human bowen disease using 25318-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human bowen disease using 25318-1-AP
Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 25318-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 25318-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human normal colon using 25318-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 25318-1-AP (Desmoplakin antibody) at dilution of 1:1600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
FC experiment of A431 using 25318-1-AP
1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human Desmoplakin (25318-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

Desmoplakin Polyclonal antibody

Desmoplakin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 25318-1-AP

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Publications(16)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA and More (1)

Synonyms

DSP, DP, DESP

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size:

-/ -

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概述

经过测试的应用
产品信息
发表文章 (16)
实验方案

Print datasheet

MSDS

经过测试的应用

Positive WB detected in	A431 cells, HepG2 cells, mouse heart tissue, BxPC-3 cells, HEK-293 cells
Positive IP detected in	BxPC-3 cells
Positive IHC detected in	rat heart tissue, human normal colon, human bowen disease, mouse heart tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells
Positive FC (Intra) detected in	A431 cells

Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:2500-1:10000
Immunofluorescence (IF)/ICC	IF/ICC : 1:750-1:3000
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

KD/KO	See 2 publications below
WB	See 13 publications below
IF	See 6 publications below
IP	See 1 publications below

产品信息

25318-1-AP targets Desmoplakin in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications	WB, IHC, IF/ICC, FC (Intra), IP, ELISA Application Description
Cited Applications	WB, IF, IP
Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Immunogen	Desmoplakin fusion protein Ag18065 种属同源性预测
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Full Name	desmoplakin
Synonyms	DSP, DP, DESP
Calculated Molecular Weight	2871 aa, 332 kDa
Observed Molecular Weight	240-330 kDa
GenBank Accession Number	BC140802
Gene Symbol	DSP
Gene ID (NCBI)	1832
RRID	AB_2880028
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P15924
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

背景介绍

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

实验方案

Product Specific Protocols
WB protocol for Desmoplakin antibody 25318-1-AP	Download protocol
IHC protocol for Desmoplakin antibody 25318-1-AP	Download protocol
IF protocol for Desmoplakin antibody 25318-1-AP	Download protocol
IP protocol for Desmoplakin antibody 25318-1-AP	Download protocol
FC protocol for Desmoplakin antibody 25318-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

发表文章

Species	Application	Title
mouse	WB	Bone Res Overexpression of Lrp5 enhanced the anti-breast cancer effects of osteocytes in bone. Authors - Shengzhi Liu View Article
mouse	WB,IF	Theranostics Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development. Authors - Min Zhang View Article
human	WB	Cancer Lett ETV4 is a theranostic target in clear cell renal cell carcinoma that promotes metastasis by activating the pro-metastatic gene FOSL1 in a PI3K-AKT dependent manner. Authors - Liang Xu View Article
mouse	WB	Cancers (Basel) Inhibition of the Growth of Breast Cancer-Associated Brain Tumors by the Osteocyte-Derived Conditioned Medium. Authors - Tomohiko Sano View Article
human	WB	J Cell Mol Med Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation. Authors - Lin-Jian Wang KD Validated View Article
human	WB	Cell Death Discov Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway. Authors - Yanlei Yang KD Validated View Article

验证数据展示

WB analysis of HepG2 using 25318-1-AP

WB analysis using 25318-1-AP

IF Staining of A431 using 25318-1-AP

IP experiment of BxPC-3 using 25318-1-AP

IHC staining of human bowen disease using 25318-1-AP

IHC staining of human bowen disease using 25318-1-AP

IHC staining of mouse heart using 25318-1-AP

IHC staining of mouse heart using 25318-1-AP

IHC staining of human normal colon using 25318-1-AP

FC experiment of A431 using 25318-1-AP

Desmoplakin Polyclonal antibody

Desmoplakin Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

经过测试的应用

推荐稀释比

发表文章中的应用

产品信息

背景介绍

实验方案

发表文章

Overexpression of Lrp5 enhanced the anti-breast cancer effects of osteocytes in bone.

Cdc42 Deficiency Leads To Epidermal Barrier Dysfunction by Regulating Intercellular Junctions and Keratinization of Epidermal Cells during Mouse Skin Development.

ETV4 is a theranostic target in clear cell renal cell carcinoma that promotes metastasis by activating the pro-metastatic gene FOSL1 in a PI3K-AKT dependent manner.

Inhibition of the Growth of Breast Cancer-Associated Brain Tumors by the Osteocyte-Derived Conditioned Medium.

Wilms' tumour 1-associating protein inhibits endothelial cell angiogenesis by m6A-dependent epigenetic silencing of desmoplakin in brain arteriovenous malformation.

Long noncoding RNA LYPLAL1-AS1 regulates adipogenic differentiation of human mesenchymal stem cells by targeting desmoplakin and inhibiting the Wnt/β-catenin pathway.