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验证数据展示

  • IF Staining of A431 using CL488-25318

    IF Staining of A431 using CL488-25318

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 Desmoplakin antibody (CL488-25318) at dilution of 1:200.

  • FC experiment of A431 using CL488-25318

    FC experiment of A431 using CL488-25318

    1X10^6 A431 cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human Desmoplakin (CL488-25318) (red), or 0.8 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 488-conjugated Desmoplakin Polyclonal antibody

Desmoplakin Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-25318

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

DSP, DP, DESP

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 25318-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-25318 targets Desmoplakin in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 Desmoplakin fusion protein Ag18065 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 desmoplakin
别名 DSP, DP, DESP
计算分子量 2871 aa, 332 kDa
观测分子量 240-330 kDa
GenBank蛋白编号BC140802
基因名称 DSP
Gene ID (NCBI) 1832
RRIDAB_3084089
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP15924
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The desmoplakin (DP) is the most abundant desmosomal proteins and is located at the cytoplasmic portion of the desmosomes which are specialized cell-cell adhesion structures abundant in tissues such as muscle and epidermis that are subjected to mechanical stress. Desmoplakin 1 and 2 (DP 1 and 2) are two splice variants sharing common C-terminal and N-terminal globular head and tail domains, but differ in the length of the rod domain that links them. This antibody detects both DP1 (280-330 kDa) and DP2 (240-260 kDa). (PMID: 16467215, 11781569)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Desmoplakin antibody CL488-25318Download protocol
FC protocol for CL Plus 488 Desmoplakin antibody CL488-25318Download protocol
Standard Protocols
Click here to view our Standard Protocols