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CoraLite® Plus 488-conjugated Destrin Monoclonal antibody

Destrin Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat, pig

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A12D9

Cat no : CL488-67657

Print datasheet

Synonyms

ACTDP, Actin depolymerizing factor, ADF, bA462D18.2, Destrin, DSN, DSTN

验证数据展示

  • FC experiment of MCF-7 using CL488-67657

    FC experiment of MCF-7 using CL488-67657

    1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human Destrin (CL488-67657, Clone:1A12D9) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC (Intra) detected inMCF-7 cells
Positive FC detected inMCF-7 cells
For other applications, we recommend the unconjugated version of this antibody, 67657-1-Ig

推荐稀释比

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67657 targets Destrin in FC (Intra) applications and shows reactivity with Human, mouse, rat, pig samples.

Tested Applications FC (Intra) Application Description
Tested Reactivity Human, mouse, rat, pig
Immunogen Destrin fusion protein Ag1402 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name destrin (actin depolymerizing factor)
Synonyms ACTDP, Actin depolymerizing factor, ADF, bA462D18.2, Destrin, DSN, DSTN
Calculated Molecular Weight 19 kDa
Observed Molecular Weight18-19 kDa
GenBank Accession NumberBC009477
Gene Symbol Destrin
Gene ID (NCBI) 11034
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP60981
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Destrin (also known as actin depolymerization factor, ADF) is an actin-binding protein contributing to cytoskeletal dynamics by promoting rapid actin filament disassembly. By regulating actin cytoskeletal reorganization it plays an important role in the formation of filopodia, which further promotes tumor cell invasion and metastasis. Destrin has been reported to be overexpressed in primary colon cancer.

实验方案

Product Specific Protocols
FC protocol for CL Plus 488 Destrin antibody CL488-67657Download protocol
Standard Protocols
Click here to view our Standard Protocols