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验证数据展示

  • Dot Blot experiment of peptide using 84329-1-RR

    Dot Blot experiment of peptide using 84329-1-RR

    Dot blot analysis was used to confirm the specificity of Di/Tri-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.

  • WB analysis using 84329-1-RR

    WB analysis using 84329-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84329-1-RR (Di/Tri-Methyl-Histone H3 (Lys36) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • WB analysis using 84329-1-RR

    WB analysis using 84329-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84329-1-RR (Di/Tri-Methyl-Histone H3 (Lys36) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • IF Staining of HeLa using 84329-1-RR

    IF Staining of HeLa using 84329-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84329-1-RR, Clone: 241139G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antibody

Di/Tri-Methyl-Histone H3 (Lys36) Recombinant Antibody for WB, IF/ICC, Dot Blot, ELISA
Cat No. 84329-1-RR
CloneNo. 241139G6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Dot Blot, ELISA

Synonyms

H3K36M3, H3K36M2/3, H3K36M2, HIST1H3A, Histone H 3

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HEK-293 cells, U-87 MG cells, U-251 cells, NIH/3T3 cells, mouse liver tissue, mouse brain tissue
Positive IF/ICC detected inHeLa cells
Positive Dot Blot detected inpeptide
Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
DOT BLOTDOT BLOT : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84329-1-RR targets Di/Tri-Methyl-Histone H3 (Lys36) in WB, IF/ICC, Dot Blot, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, IF/ICC, Dot Blot, ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms H3K36M3, H3K36M2/3, H3K36M2, HIST1H3A, Histone H 3
Observed Molecular Weight15 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
RRIDAB_3671870
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.

实验方案

Product Specific Protocols
WB protocol for Di/Tri-Methyl-Histone H3 (Lys36) antibody 84329-1-RRDownload protocol
IF protocol for Di/Tri-Methyl-Histone H3 (Lys36) antibody 84329-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols