• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

Di/Tri-Methyl-Histone H3 (Lys36) Recombinant antibody, PBS Only

Di/Tri-Methyl-Histone H3 (Lys36) Uni-rAbTM Recombinant Antibody for WB, IF/ICC, Dot Blot, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Dot Blot, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241139G6

Cat no : 84329-1-PBS

Print datasheet

Synonyms

HIST1H3A, 241139G6, Histone-H3, HistoneH3, Histone H3

验证数据展示

  • Dot Blot experiment of peptide using 84329-1-RR (same clone as 84329-1-PBS)

    Dot Blot experiment of peptide using 84329-1-RR (same clone as 84329-1-PBS)

    Dot blot analysis was used to confirm the specificity of Di/Tri-Methyl-Histone H3 (Lys36) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row. This data was developed using the same antibody clone with 84329-1-PBS in a different storage buffer formulation.

  • WB analysis using 84329-1-RR (same clone as 84329-1-PBS)

    WB analysis using 84329-1-RR (same clone as 84329-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84329-1-RR (Di/Tri-Methyl-Histone H3 (Lys36) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84329-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84329-1-RR (same clone as 84329-1-PBS)

    IF Staining of HeLa using 84329-1-RR (same clone as 84329-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HIST1H3A antibody (84329-1-RR, Clone: 241139G6 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84329-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy Phospho-Histone H3 (Ser10) Ready-To-Use IHC Kit. Phospho-Histone H3 (Ser10) primary antibody included.

产品信息

84329-1-PBS targets Di/Tri-Methyl-Histone H3 (Lys36) in WB, IF/ICC, Dot Blot, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB, IF/ICC, Dot Blot, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Peptide 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name histone cluster 1, H3a
Synonyms HIST1H3A, 241139G6, Histone-H3, HistoneH3, Histone H3
Observed Molecular Weight15 kDa
GenBank Accession NumberBC066245
Gene Symbol HIST1H3A
Gene ID (NCBI) 8350
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP68431
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histones are small, highly basic proteins that consist of a globular domain with unstructured N- and C-terminal tails protruding from the main structure. Histone H3 is one of the five main histones that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. In addition to their role in DNA compartmentalization, histones also play crucial roles in various biologic processes, including gene expression and regulation, DNA repair, chromatin condensation, cell cycle progression, chromosome segregation, and apoptosis. The ability of histones to regulate chromatin dynamics primarily originates from various posttranslational modifications carried out by histone-modifying enzymes.