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Drebrin Polyclonal antibody

Drebrin Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA and More (1)

Conjugate

Unconjugated

Cat no : 25770-1-AP

Print datasheet

Synonyms

D0S117E, DBN1, Drebrin, drebrin 1

验证数据展示

  • WB analysis using 25770-1-AP

    WB analysis using 25770-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 25770-1-AP

    WB analysis of K-562 using 25770-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 25770-1-AP (Drebrin Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • IF Staining of SH-SY5Y using 25770-1-AP

    IF Staining of SH-SY5Y using 25770-1-AP

    Immunofluorescent analysis of SH-SY5Y cells using 25770-1-AP (Drebrin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  • IF Staining of MCF-7 using 25770-1-AP

    IF Staining of MCF-7 using 25770-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using Drebrin antibody (25770-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inHeLa cells, mouse brain tissue, SH-SY5Y cells, K-562 cells, Neuro-2a cells, Jurkat cells
Positive IF/ICC detected inMCF-7 cells, SH-SY5Y cells, HepG2 cells
Planning an IHC experiment? We recommend our IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit. DBN1/Drebrin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

IFSee 1 publications below

产品信息

25770-1-AP targets Drebrin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Cited ApplicationsIF
Tested Reactivity human, mouse
Cited Reactivityhuman
Immunogen Drebrin fusion protein Ag22634 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name drebrin 1
Synonyms D0S117E, DBN1, Drebrin, drebrin 1
Observed Molecular Weight 100-130 kDa
GenBank Accession NumberBC007567
Gene Symbol Drebrin
Gene ID (NCBI) 1627
ENSEMBL Gene IDENSG00000113758
RRIDAB_2880234
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ16643
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.

实验方案

Product Specific Protocols
WB protocol for Drebrin antibody 25770-1-APDownload protocol
IF protocol for Drebrin antibody 25770-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIF

Int J Mol Sci

Drebrin Regulates Acetylcholine Receptor Clustering and Organization of Microtubules at the Postsynaptic Machinery

Authors - Paloma Alvarez-Suarez
View Article