IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit
DBN1/Drebrin Ready-to-use reagent kit for IHC.
Reactivity
Human, Mouse, Rat
Sample Type
FFPE tissue
Cat No : KHC0848
Synonyms
D0S117E, DBN1, DKFZp434D064, Drebrin, drebrin 1
Validation Data Gallery
Product Information
KHC0848 is a ready-to-use IHC kit for staining of DBN1/Drebrin. The kit provides all reagents, from antigen retrieval to cover slip mounting, that require little to no diluting or handling prior to use. Simply apply the reagents to your sample slide according to the protocol and you're steps away from obtaining high-quality IHC data.
| Product name | IHCeasy DBN1/Drebrin Ready-To-Use IHC Kit |
| Sample type | FFPE tissue |
| Assay type | Immunohistochemistry |
| Primary antibody type | Mouse Monoclonal |
| Secondary antibody type | Polymer-HRP-Goat anti-Mouse |
| Reactivity | Human, Mouse, Rat |
Kit components
| Component | Size | Concentration |
|---|---|---|
| Antigen Retrieval Buffer | 100 mL | 50× |
| Washing Buffer | 100 mL ×2 | 20× |
| Blocking Buffer | 5 mL | RTU |
| Primary Antibody | 5 mL | RTU |
| Secondary Antibody | 5 mL | RTU |
| Chromogen Component A | 0.2 mL | RTU |
| Chromogen Component B | 4 mL | RTU |
| Signal Enhancer | 5 mL | RTU |
| Counter Staining Reagent | 5 mL | RTU |
| Mounting Media | 5 mL | RTU |
| Datasheet | 1 Copy | |
| Manual | 1 Copy |
Background Information
Drebrin (DBN1) is an actin binding and stabilizing protein with roles in endocytosis, formation of dendrite spines in neurons and coordinating cell-cell synapses in immune cells. Preferentially expressed in the brain, it is highly localized in dendritic spines and regulates spine shapes. It has two isoforms that are splice variants, with drebrin A being expressed in neural cells, and drebrin E is present in several other cell types. Recently it has been reported that circulating DBN1 levels correlated with retinal nerve fiber layer defect and may reflect the severity of retinal ganglion cells injury in glaucoma patients.
Properties
| Storage Instructions | All the reagents are stored at 2-8°C. The kit is stable for 6 months from the date of receipt. |
| Synonyms | D0S117E, DBN1, DKFZp434D064, Drebrin, drebrin 1 |
