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  • Featured Product
  • KD/KO Validated

Dystroglycan Monoclonal antibody, PBS Only

Dystroglycan Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B1G12

Cat no : 66735-1-PBS

Print datasheet

Synonyms

156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan

验证数据展示

  • WB analysis using 66735-1-Ig (same clone as 66735-1-PBS)

    WB analysis using 66735-1-Ig (same clone as 66735-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66735-1-Ig (Dystroglycan antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAG1 antibody included
  • IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    IHC staining of mouse heart using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66735-1-Ig (Dystroglycan antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with DAG1 antibody included
  • IF Staining of HeLa using 66735-1-Ig (same clone as 66735-1-PBS)

    IF Staining of HeLa using 66735-1-Ig (same clone as 66735-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Dystroglycan antibody (66735-1-Ig, Clone: 2B1G12 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66735-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy DAG1 Ready-To-Use IHC Kit. DAG1 primary antibody included.

产品信息

66735-1-PBS targets Dystroglycan in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen Dystroglycan fusion protein Ag27222 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dystroglycan 1 (dystrophin-associated glycoprotein 1)
Synonyms 156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 43 kDa, 30 kDa
GenBank Accession NumberBC012740
Gene Symbol Dystroglycan
Gene ID (NCBI) 1605
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ14118
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).