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- KD/KO Validated
Dystroglycan Monoclonal antibody
Dystroglycan Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig and More (1)
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
2B1G12
验证数据展示
经过测试的应用
Positive WB detected in | A549 cells, NCI-H1299 cells, HeLa cells, HepG2 cells, pig brain tissue, rat brain tissue, mouse brain |
Positive IHC detected in | mouse heart tissue, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
FC | See 1 publications below |
产品信息
66735-1-Ig targets Dystroglycan in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | Human, Mouse, Rat, Pig |
Cited Reactivity | human |
Immunogen | Dystroglycan fusion protein Ag27222 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | dystroglycan 1 (dystrophin-associated glycoprotein 1) |
Synonyms | 156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan |
Calculated Molecular Weight | 97 kDa |
Observed Molecular Weight | 43 kDa, 30 kDa |
GenBank Accession Number | BC012740 |
Gene Symbol | Dystroglycan |
Gene ID (NCBI) | 1605 |
RRID | AB_2882085 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q14118 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for Dystroglycan antibody 66735-1-Ig | Download protocol |
IHC protocol for Dystroglycan antibody 66735-1-Ig | Download protocol |
IF protocol for Dystroglycan antibody 66735-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Proc Natl Acad Sci U S A CACNA1S haploinsufficiency confers resistance to New World arenavirus infection. |