CoraLite® Plus 488-conjugated Dystroglycan Monoclonal antibody
Dystroglycan Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, Mouse, Rat, Pig
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2B1G12
验证数据展示
经过测试的应用
| Positive IF detected in | mouse heart tissue |
Planning an IHC experiment? We recommend our IHCeasy DAG1 Ready-To-Use IHC Kit. DAG1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66735-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL488-66735 targets Dystroglycan in IF applications and shows reactivity with Human, Mouse, Rat, Pig samples.
| Tested Applications | IF-P Application Description |
| Tested Reactivity | Human, Mouse, Rat, Pig |
| Immunogen | Dystroglycan fusion protein Ag27222 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | dystroglycan 1 (dystrophin-associated glycoprotein 1) |
| Synonyms | 156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan |
| Calculated Molecular Weight | 97 kDa |
| Observed Molecular Weight | 43 kDa, 30 kDa |
| GenBank Accession Number | BC012740 |
| Gene Symbol | DAG1 |
| Gene ID (NCBI) | 1605 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q14118 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Dystroglycan antibody CL488-66735 | Download protocol |
| FC protocol for CL Plus 488 Dystroglycan antibody CL488-66735 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
