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CoraLite® Plus 488-conjugated Dystroglycan Monoclonal antibody

Dystroglycan Monoclonal Antibody for IF-P

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B1G12

Cat no : CL488-66735

Print datasheet

Synonyms

156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan



经过测试的应用

Positive IF-P detected inmouse heart tissue
Planning an IHC experiment? We recommend our IHCeasy DAG1 Ready-To-Use IHC Kit. DAG1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66735-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-66735 targets Dystroglycan in IF-P applications and shows reactivity with Human, Mouse, Rat, Pig samples.

Tested Applications IF-P Application Description
Tested Reactivity Human, Mouse, Rat, Pig
Immunogen Dystroglycan fusion protein Ag27222 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name dystroglycan 1 (dystrophin-associated glycoprotein 1)
Synonyms 156DAG, A3a, AGRNR, Alpha DG Beta dystroglycan, Beta DG, DAG, DAG1, Dystroglycan
Calculated Molecular Weight 97 kDa
Observed Molecular Weight 43 kDa, 30 kDa
GenBank Accession NumberBC012740
Gene Symbol Dystroglycan
Gene ID (NCBI) 1605
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ14118
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Dystroglycan, also known as DAG1 or DG, was originally isolated from skeletal muscle as an integral membrane component of the dystrophin-glycoprotein complex (DGC). In addition to skeletal muscle, dystroglycan is strongly expressed in heart and smooth muscle, as well as many non-muscle tissues including brain and peripheral nerve (PMID: 12556455). The dystroglycan is involved in a number of processes including laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Dystroglycan consists of two subunits (alpha and beta), which are translated from a single mRNA as a propeptide that is proteolytically cleaved into two noncovalently associated proteins (PMID: 16410545). Alpha-dystroglycan is a 156-kDa extracellular peripheral glycoprotein, while beta-dystroglycan is a 43-kDa transmembrane protein (PMID: 9858474). The 43-kDa beta-dystroglycan can be cleaved into a ~30-kDa form (PMID: 14678802; 18458097; 17255331).

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 Dystroglycan antibody CL488-66735Download protocol
FC protocol for CL Plus 488 Dystroglycan antibody CL488-66735Download protocol
Standard Protocols
Click here to view our Standard Protocols