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E-cadherin Recombinant antibody, PBS Only (Capture)

E-cadherin Recombinant Antibody for WB, IF, Indirect ELISA, Cytometric bead array

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, Indirect ELISA, Cytometric bead array

Conjugate

Unconjugated

CloneNo.

240159C10

Cat No : 80541-5-PBS

Print datasheet

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin

验证数据展示

  • WB analysis using 80541-5-RR (same clone as 80541-5-PBS)

    WB analysis using 80541-5-RR (same clone as 80541-5-PBS)

    Caco-2 cells were subjected to SDS PAGE followed by western blot with 80541-5-RR (E-cadherin antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80541-5-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 80541-5-RR (same clone as 80541-5-PBS)

    IF Staining of MCF-7 using 80541-5-RR (same clone as 80541-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (80541-5-RR, Clone: 240159C10 ) at dilution of 1:250 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80541-5-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00187-1

    Cytometric bead array standard curve of MP00187-1

    Cytometric bead array standard curve ofMP00187-1, E-cadherin Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 80541-5-PBS. Detection antibody: 80541-2-PBS. Standard: Ag14973. Range: 0.625-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

产品信息

80541-5-PBS targets E-cadherin as part of a matched antibody pair:

MP00187-1: 80541-5-PBS capture and 80541-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IF, Indirect ELISA, Cytometric bead array Application Description
Tested Reactivity Human
Immunogen E-cadherin fusion protein Ag14973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Synonyms Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin
Calculated Molecular Weight 882 aa, 97 kDa
GenBank Accession NumberBC141838
Gene Symbol CDH1
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12830
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.