E-cadherin Recombinant antibody
E-cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240159C10
验证数据展示
经过测试的应用
Positive WB detected in | HCT 116 cells, Caco-2 cells, A431 cells, T-47D cells, MCF-7 cells |
Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | MCF-7 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80541-5-RR targets E-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | E-cadherin fusion protein Ag14973 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
Synonyms | CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1 |
Calculated Molecular Weight | 882 aa, 97 kDa |
Observed Molecular Weight | 135 kDa |
GenBank Accession Number | BC141838 |
Gene Symbol | E-cadherin |
Gene ID (NCBI) | 999 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P12830 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
---|---|
WB protocol for E-cadherin antibody 80541-5-RR | Download protocol |
IHC protocol for E-cadherin antibody 80541-5-RR | Download protocol |
IF protocol for E-cadherin antibody 80541-5-RR | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |