E-cadherin Recombinant antibody
E-cadherin Recombinant Antibody for WB, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
240159C10
验证数据展示
经过测试的应用
Positive WB detected in | Caco-2 cells, A431 cells, T-47D cells |
Positive IF detected in | MCF-7 cells |
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunofluorescence (IF) | IF : 1:125-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
80541-5-RR targets E-cadherin in WB, IF, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IF/ICC, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | E-cadherin fusion protein Ag14973 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | cadherin 1, type 1, E-cadherin (epithelial) |
Synonyms | Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin |
Calculated Molecular Weight | 882 aa, 97 kDa |
Observed Molecular Weight | 135 kDa |
GenBank Accession Number | BC141838 |
Gene Symbol | CDH1 |
Gene ID (NCBI) | 999 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P12830 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.
实验方案
Product Specific Protocols | |
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WB protocol for E-cadherin antibody 80541-5-RR | Download protocol |
IF protocol for E-cadherin antibody 80541-5-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |