E-cadherin Recombinant antibody

E-cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 80541-5-RR

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CloneNo. 240159C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, ELISA

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -


经过测试的应用

Positive WB detected inHCT 116 cells, Caco-2 cells, A431 cells, T-47D cells, MCF-7 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inMCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

80541-5-RR targets E-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Cited ApplicationsWB
Tested Reactivity human
Cited Reactivityhuman
Immunogen E-cadherin fusion protein Ag14973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Synonyms CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1
Calculated Molecular Weight 882 aa, 97 kDa
Observed Molecular Weight135 kDa
GenBank Accession NumberBC141838
Gene Symbol E-cadherin
Gene ID (NCBI) 999
RRIDAB_3670479
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP12830
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.

实验方案

Product Specific Protocols
WB protocol for E-cadherin antibody 80541-5-RRDownload protocol
IHC protocol for E-cadherin antibody 80541-5-RRDownload protocol
IF protocol for E-cadherin antibody 80541-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Sci Rep

Hypoxia and lipid metabolism related genes drive proliferation migration and immune infiltration mechanisms in colorectal cancer subtyping

Authors - Shansong Huang
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