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E-cadherin Recombinant antibody, PBS Only

E-cadherin Recombinant Antibody for WB, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

WB, IF, ELISA

Conjugate

Unconjugated

CloneNo.

240159A1

Cat No : 80541-7-PBS

Print datasheet

Synonyms

Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin

验证数据展示

  • WB analysis using 80541-7-RR (same clone as 80541-7-PBS)

    WB analysis using 80541-7-RR (same clone as 80541-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80541-7-RR (E-cadherin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80541-7-PBS in a different storage buffer formulation.

  • IF Staining of MCF-7 using 80541-7-RR (same clone as 80541-7-PBS)

    IF Staining of MCF-7 using 80541-7-RR (same clone as 80541-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (80541-7-RR, Clone: 240159A1 ) at dilution of 1:160 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80541-7-PBS in a different storage buffer formulation.

  • Affinity experiments of 80541-7-RR

    Affinity experiments of 80541-7-RR

    Biolayer interferometry (BLl) kinetic assays of 80541-7-RR against Human E-cadherin were performed. The affinity constant is below 1 pM.

Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

产品信息

The immunogen of 80541-7-PBS is E-cadherin Fusion Protein expressed in E. coli.

Tested Applications WB, IF, ELISA Application Description
Tested Reactivity Human
Immunogen E-cadherin fusion protein Ag14973 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name cadherin 1, type 1, E-cadherin (epithelial)
Synonyms Arc 1, Cadherin 1, CAM 120/80, CD324, CDH1, CDHE, E cadherin, ECAD, Epithelial cadherin, LCAM, UVO, Uvomorulin
Calculated Molecular Weight 882 aa, 97 kDa
GenBank Accession NumberBC141838
Gene Symbol CDH1
Gene ID (NCBI) 999
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDP12830
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.