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验证数据展示

  • WB analysis using 80541-7-RR (same clone as 80541-7-PBS)

    WB analysis using 80541-7-RR (same clone as 80541-7-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80541-7-RR (E-cadherin antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80541-7-PBS in a different storage buffer formulation.

  • IHC staining of human stomach cancer using 80541-7-RR (same clone as 80541-7-PBS)

    IHC staining of human stomach cancer using 80541-7-RR (same clone as 80541-7-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 80541-7-RR (E-cadherin antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 80541-7-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with E-cadherin antibody included
  • IF Staining of MCF-7 using 80541-7-RR (same clone as 80541-7-PBS)

    IF Staining of MCF-7 using 80541-7-RR (same clone as 80541-7-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using E-cadherin antibody (80541-7-RR, Clone: 240159A1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 80541-7-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80541-7-RR

    Affinity and Kinetic Characterization of 80541-7-RR

    Biolayer interferometry (BLl) kinetic assays of 80541-7-RR against Human E-cadherin were performed. The affinity constant is below 1 pM.

E-cadherin Recombinant antibody, PBS Only

E-cadherin Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 80541-7-PBS

产品说明书

CloneNo. 240159A1

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, ELISA

别名

CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy E-cadherin Ready-To-Use IHC Kit. E-cadherin primary antibody included.

产品信息

80541-7-PBS targets E-cadherin in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原 E-cadherin fusion protein Ag14973 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 cadherin 1, type 1, E-cadherin (epithelial)
别名 CD324, CAM 120/80, Cadherin-1, Cadherin 1, Arc 1
计算分子量 882 aa, 97 kDa
观测分子量135 kDa
GenBank蛋白编号BC141838
基因名称 E-cadherin
Gene ID (NCBI) 999
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purfication
UNIPROT IDP12830
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. E-cadherin (epithelial cadherin), also known as CDH1 (cadherin 1) or CAM 120/80, is a classical member of the cadherin superfamily which also include N-, P-, R-, and B-cadherins. E-cadherin is expressed on the cell surface in most epithelial tissues. The extracellular region of E-cadherin establishes calcium-dependent homophilic trans binding, providing specific interaction with adjacent cells, while the cytoplasmic domain is connected to the actin cytoskeleton through the interaction with p120-, α-, β-, and γ-catenin (plakoglobin). E-cadherin is important in the maintenance of the epithelial integrity, and is involved in mechanisms regulating proliferation, differentiation, and survival of epithelial cell. E-cadherin may also play a role in tumorigenesis. It is considered to be an invasion suppressor protein and its loss is an indicator of high tumor aggressiveness.