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E2F4 Monoclonal antibody, PBS Only

E2F4 Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D1E1

Cat no : 67812-1-PBS

Print datasheet

Synonyms

E2F 4, E2F4, Transcription factor E2F4

验证数据展示

  • WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

  • WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    WB analysis using 67812-1-Ig (same clone as 67812-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67812-1-Ig (E2F4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 67812-1-PBS in a different storage buffer formulation.

产品信息

67812-1-PBS targets E2F4 in WB, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity Human, mouse, rat
Immunogen E2F4 fusion protein Ag28533 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name E2F transcription factor 4, p107/p130-binding
Synonyms E2F 4, E2F4, Transcription factor E2F4
Calculated Molecular Weight 44 kDa
GenBank Accession NumberBC033180
Gene Symbol E2F4
Gene ID (NCBI) 1874
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16254
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Transcription factor E2F4 (E2F4) is a transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. E2F-4 binds with high affinity to RBL1 and RBL2. In some instances, can also bind RB protein.The observed molecular weight of 60-70 kDa is the phosphorylation of E2F4 protein.