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验证数据展示

  • WB analysis using 27245-1-AP

    WB analysis using 27245-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27245-1-AP (ECE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

ECE1 Polyclonal antibody

ECE1 Polyclonal Antibody for WB, ELISA
Cat No. 27245-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, ELISA

别名

ECE, ECE 1, ECE1, endothelin converting enzyme 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, HUVEC cells, MCF-7 cells
Planning an IHC experiment? We recommend our IHCeasy ECE1 Ready-To-Use IHC Kit. ECE1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

27245-1-AP targets ECE1 in WB, ELISA applications and shows reactivity with human samples.

经测试应用 WB, ELISA Application Description
经测试反应性 human
免疫原 ECE1 fusion protein Ag26124 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 endothelin converting enzyme 1
别名 ECE, ECE 1, ECE1, endothelin converting enzyme 1
观测分子量87-110 kDa
GenBank蛋白编号BC126257
基因名称 ECE1
Gene ID (NCBI) 1889
RRIDAB_3085941
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP42892
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ECE1, Endothelin-converting enzyme 1, also known as ECE. It is located in cell membrane, and it has 4 isoforms. All isoforms are expressed in umbilical vein endothelial cells, polynuclear neutrophils, fibroblasts, atrium cardiomyocytes and ventricles. Isoforms A, B and C are also expressed in placenta, lung, heart, adrenal gland and phaeochromocytoma; isoforms A and C in liver, testis and small intestine; isoform B, C and D in endothelial cells and umbilical vein smooth muscle cells; isoforms C and D in saphenous vein cells, and isoform C in kidney (PMID: 9396733). The protein encoded by this gene is involved in proteolytic processing of endothelin precursors to biologically active peptides. Mutations in this gene are associated with Hirschsprung disease, cardiac defects and autonomic dysfunction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. The calculated molecular weight of ECE1 is 87 kDa, as a result of glycosylation, the apparent molecular mass of ECE1 is approximately 87-110 kDa.

实验方案

Product Specific Protocols
WB protocol for ECE1 antibody 27245-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols