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ECH1 Monoclonal antibody

ECH1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

1C9G5

Cat no : 68139-1-Ig

Print datasheet

Synonyms

ECH1, HPXEL

验证数据展示

  • WB analysis using 68139-1-Ig

    WB analysis using 68139-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

  • IHC staining of human liver cancer using 68139-1-Ig

    IHC staining of human liver cancer using 68139-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECH1 antibody included
  • IHC staining of human liver cancer using 68139-1-Ig

    IHC staining of human liver cancer using 68139-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECH1 antibody included
  • IF Staining of HepG2 using 68139-1-Ig

    IF Staining of HepG2 using 68139-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, HEK-293 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy ECH1 Ready-To-Use IHC Kit. ECH1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68139-1-Ig targets ECH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen ECH1 fusion protein Ag32661 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name enoyl Coenzyme A hydratase 1, peroxisomal
Synonyms ECH1, HPXEL
Calculated Molecular Weight 328 aa, 36 kDa
Observed Molecular Weight 33-36 kDa
GenBank Accession NumberBC017408
Gene Symbol ECH1
Gene ID (NCBI) 1891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13011
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for ECH1 antibody 68139-1-IgDownload protocol
IHC protocol for ECH1 antibody 68139-1-IgDownload protocol
IF protocol for ECH1 antibody 68139-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols