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ECH1 Monoclonal antibody, PBS Only

ECH1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

1C9G5

Cat No : 68139-1-PBS

Print datasheet

Synonyms

ECH1, HPXEL

验证数据展示

  • WB analysis using 68139-1-Ig (same clone as 68139-1-PBS)

    WB analysis using 68139-1-Ig (same clone as 68139-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68139-1-Ig (ECH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

    IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECH1 antibody included
  • IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

    IHC staining of human liver cancer using 68139-1-Ig (same clone as 68139-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 68139-1-Ig (ECH1 antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECH1 antibody included
  • IF Staining of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

    IF Staining of HepG2 using 68139-1-Ig (same clone as 68139-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using ECH1 antibody (68139-1-Ig, Clone: 1C9G5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68139-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ECH1 Ready-To-Use IHC Kit. ECH1 primary antibody included.

产品信息

68139-1-PBS targets ECH1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human
Immunogen ECH1 fusion protein Ag32661 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name enoyl Coenzyme A hydratase 1, peroxisomal
Synonyms ECH1, HPXEL
Calculated Molecular Weight 328 aa, 36 kDa
Observed Molecular Weight 33-36 kDa
GenBank Accession NumberBC017408
Gene Symbol ECH1
Gene ID (NCBI) 1891
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ13011
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.