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  • Featured Product
  • KD/KO Validated

ECHS1 Monoclonal antibody, PBS Only

ECHS1 Monoclonal Antibody for WB,IP,Indirect ELISA,IHC,IF

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB,IP,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

2B9D5

Cat No : 66117-1-PBS

Print datasheet

Synonyms

ECHS1, Enoyl CoA hydratase 1, SCEH

验证数据展示

  • WB analysis of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    WB analysis of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    WB result of ECHS1 antibody (66117-1-Ig, 1:20,000) with si-Control and si-ECHS1 transfected HepG2 cells. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • WB analysis using 66117-1-Ig (same clone as 66117-1-PBS)

    WB analysis using 66117-1-Ig (same clone as 66117-1-PBS)

    Various cells were subjected to SDS PAGE followed by western blot with 66117-1-Ig (ECHS1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECHS1 antibody included
  • IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    IHC staining of human prostate cancer using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66117-1-Ig (ECHS1 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECHS1 antibody included
  • IP experiment of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    IP experiment of HepG2 using 66117-1-Ig (same clone as 66117-1-PBS)

    IP result of anti-ECHS1 (IP:66117-1-Ig, 3ug; Detection:66117-1-Ig 1:1000) with HepG2 cells lysate 3600ug. This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66117-1-Ig (same clone as 66117-1-PBS)

    IF Staining of HeLa using 66117-1-Ig (same clone as 66117-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using ECHS1 antibody (66117-1-Ig, Clone: 2B9D5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66117-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.

产品信息

66117-1-PBS targets ECHS1 in WB, IP, IF, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Applications WB,IP,Indirect ELISA,IHC,IF Application Description
Tested Reactivity human
Immunogen ECHS1 fusion protein Ag16775 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Synonyms ECHS1, Enoyl CoA hydratase 1, SCEH
Calculated Molecular Weight 31 kDa
Observed Molecular Weight 31 kDa
GenBank Accession NumberBC008906
Gene Symbol ECHS1
Gene ID (NCBI) 1892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30084
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered as a characteristic feature of mitochondria dysfunction. (23275097, 23235493)