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ECHS1 Recombinant antibody, PBS Only

ECHS1 Recombinant Antibody for WB,Indirect ELISA,IHC,IF

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

WB,Indirect ELISA,IHC,IF

Conjugate

Unconjugated

CloneNo.

5G5

Cat No : 81611-1-PBS

Print datasheet

Synonyms

ECHS1, Enoyl CoA hydratase 1, SCEH

验证数据展示

  • WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

    WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

  • WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

    WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 81611-1-RR (same clone as 81611-1-PBS)

    IHC staining of human prostate cancer using 81611-1-RR (same clone as 81611-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECHS1 antibody included
  • IF Staining of HeLa using 81611-1-RR (same clone as 81611-1-PBS)

    IF Staining of HeLa using 81611-1-RR (same clone as 81611-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ECHS1 antibody (81611-1-RR, Clone: 5G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.

产品信息

81611-1-PBS targets ECHS1 in WB, IF, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ECHS1 fusion protein Ag1842 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Synonyms ECHS1, Enoyl CoA hydratase 1, SCEH
Calculated Molecular Weight 31 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC008906
Gene Symbol ECHS1
Gene ID (NCBI) 1892
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30084
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493)