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验证数据展示

  • WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

    WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

  • WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

    WB analysis using 81611-1-RR (same clone as 81611-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81611-1-RR (ECHS1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

  • WB analysis of MCF-7 using 81611-1-RR (same clone as 81611-1-PBS)

    WB analysis of MCF-7 using 81611-1-RR (same clone as 81611-1-PBS)

    WB result of ECHS1 antibody (81611-1-RR; 1:15000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ECHS1 transfected MCF-7 cells. This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

  • IHC staining of human prostate cancer using 81611-1-RR (same clone as 81611-1-PBS)

    IHC staining of human prostate cancer using 81611-1-RR (same clone as 81611-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 81611-1-RR (ECHS1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECHS1 antibody included
  • IF Staining of HeLa using 81611-1-RR (same clone as 81611-1-PBS)

    IF Staining of HeLa using 81611-1-RR (same clone as 81611-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using ECHS1 antibody (81611-1-RR, Clone: 5G5 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 81611-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81611-1-RR

    Affinity and Kinetic Characterization of 81611-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81611-1-RR against Human ECHS1 were performed. The affinity constant is 95.5 pM.

  • Featured Product
  • KD/KO验证

ECHS1 Recombinant antibody, PBS Only

ECHS1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 81611-1-PBS

产品说明书

CloneNo. 5G5

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.

产品信息

81611-1-PBS targets ECHS1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 ECHS1 fusion protein Ag1842 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
别名 mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17
计算分子量 31 kDa
观测分子量30 kDa
GenBank蛋白编号BC008906
基因名称 ECHS1
Gene ID (NCBI) 1892
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP30084
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493)