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ECHS1 Recombinant antibody
ECHS1 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, ELISA
Conjugate
Unconjugated
CloneNo.
5G5
验证数据展示
经过测试的应用
Positive WB detected in | HeLa cells, MCF-7 cells, mouse liver tissue, L02 cells, Caco-2 cells, HepG2 cells, HEK-293 cells, PC-3 cells, rat liver tissue |
Positive IHC detected in | human prostate cancer tissue, human colon tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | HeLa cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81611-1-RR targets ECHS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | WB, IHC, IF/ICC, ELISA Application Description |
Tested Reactivity | human, mouse, rat |
Immunogen | ECHS1 fusion protein Ag1842 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | enoyl Coenzyme A hydratase, short chain, 1, mitochondrial |
Synonyms | mECH, Enoyl-CoA hydratase, mitochondrial, Enoyl-CoA hydratase 1, ECHM, EC:4.2.1.17 |
Calculated Molecular Weight | 31 kDa |
Observed Molecular Weight | 30 kDa |
GenBank Accession Number | BC008906 |
Gene Symbol | ECHS1 |
Gene ID (NCBI) | 1892 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P30084 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for ECHS1 antibody 81611-1-RR | Download protocol |
IHC protocol for ECHS1 antibody 81611-1-RR | Download protocol |
IF protocol for ECHS1 antibody 81611-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |