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CoraLite® Plus 488-conjugated ECHS1 Recombinant antibody

ECHS1 Recombinant Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5G5

Cat No : CL488-81611

Print datasheet

Synonyms

ECHS1, Enoyl CoA hydratase 1, SCEH

验证数据展示

  • IF Staining of HeLa using CL488-81611

    IF Staining of HeLa using CL488-81611

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 ECHS1 antibody (CL488-81611, Clone: 5G5 ) at dilution of 1:200, CL594-Phalloidin (red).

经过测试的应用

Positive IF detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy ECHS1 Ready-To-Use IHC Kit. ECHS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 81611-1-PBS

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-81611 targets ECHS1 in IF applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, Mouse, Rat
Immunogen ECHS1 fusion protein Ag1842 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name enoyl Coenzyme A hydratase, short chain, 1, mitochondrial
Synonyms ECHS1, Enoyl CoA hydratase 1, SCEH
Calculated Molecular Weight 31 kDa
Observed Molecular Weight30 kDa
GenBank Accession NumberBC008906
Gene Symbol ECHS1
Gene ID (NCBI) 1892
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP30084
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493)

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 ECHS1 antibody CL488-81611Download protocol
Standard Protocols
Click here to view our Standard Protocols