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验证数据展示

  • WB analysis of MCF-7 using 66023-1-Ig

    WB analysis of MCF-7 using 66023-1-Ig

    MCF7 cells were subjected to SDS PAGE followed by western blot with 66023-1-Ig (ECM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66023-1-Ig

    IHC staining of human breast cancer using 66023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 10x lens).

    Ready-to-use IHC kits available with ECM1 antibody included
  • IHC staining of human breast cancer using 66023-1-Ig

    IHC staining of human breast cancer using 66023-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 66023-1-Ig(ECM1 antibody) at dilution of 1:50 (under 40x lens).

    Ready-to-use IHC kits available with ECM1 antibody included

ECM1 Monoclonal antibody

ECM1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66023-1-Ig

Print datasheet

CloneNo. 6A12B3

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

ECM1, extracellular matrix protein 1, Secretory component p85

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inMCF7 cells, COLO 320 cells, human heart tissue
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ECM1 Ready-To-Use IHC Kit. ECM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66023-1-Ig targets ECM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen ECM1 fusion protein Ag17955 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name extracellular matrix protein 1
Synonyms ECM1, extracellular matrix protein 1, Secretory component p85
Calculated Molecular Weight 540 aa, 61 kDa
Observed Molecular Weight 61 kDa
GenBank Accession NumberBC023505
Gene Symbol ECM1
Gene ID (NCBI) 1893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16610
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Extracellular matrix protein 1 (ECM1) is a glycoprotein involved in a number of biological processes such as such as bone formation, skin differentiation, cell proliferation, and promotes angiostasis. Pathologically, ECM1 contributes to the formation and metastasis of several types of cancer including breast, thyroid and hepatocellular cancers.(21128013) It inhibits MMP9 proteolytic activity.