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ECM1 Monoclonal antibody

ECM1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2D1A3

Cat no : 68472-1-Ig

Print datasheet

Synonyms

Secretory component p85, extracellular matrix protein 1, 2D1A3

验证数据展示

  • WB analysis using 68472-1-Ig

    WB analysis using 68472-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of human plasma using 68472-1-Ig

    WB analysis of human plasma using 68472-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 68472-1-Ig

    WB analysis of human placenta using 68472-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68472-1-Ig

    WB analysis of A431 using 68472-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 68472-1-Ig

    WB analysis of K-562 using 68472-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 68472-1-Ig

    IHC staining of human skin cancer using 68472-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68472-1-Ig (ECM1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECM1 antibody included

经过测试的应用

Positive WB detected inhTERT-RPE1 cells, A431 cells, K-562 cells, human placenta tissue, human plasma, THP-1 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ECM1 Ready-To-Use IHC Kit. ECM1 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68472-1-Ig targets ECM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name extracellular matrix protein 1
Synonyms Secretory component p85, extracellular matrix protein 1, 2D1A3
Calculated Molecular Weight 540 aa, 61 kDa
Observed Molecular Weight60-80 kDa
GenBank Accession NumberBC023505
Gene Symbol ECM1
Gene ID (NCBI) 1893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16610
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.

实验方案

Product Specific Protocols
WB protocol for ECM1 antibody 68472-1-IgDownload protocol
IHC protocol for ECM1 antibody 68472-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols