验证数据展示
经过测试的应用
Positive WB detected in | hTERT-RPE1 cells, A431 cells, K-562 cells, human placenta tissue, human plasma, THP-1 cells |
Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:2000-1:8000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
68472-1-Ig targets ECM1 in WB, IHC, ELISA applications and shows reactivity with human samples.
Tested Applications | WB, IHC, ELISA Application Description |
Tested Reactivity | human |
Immunogen | Recombinant protein 种属同源性预测 |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Full Name | extracellular matrix protein 1 |
Synonyms | Secretory component p85, extracellular matrix protein 1, 2D1A3 |
Calculated Molecular Weight | 540 aa, 61 kDa |
Observed Molecular Weight | 60-80 kDa |
GenBank Accession Number | BC023505 |
Gene Symbol | ECM1 |
Gene ID (NCBI) | 1893 |
RRID | AB_3085183 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein G purification |
UNIPROT ID | Q16610 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.
实验方案
Product Specific Protocols | |
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WB protocol for ECM1 antibody 68472-1-Ig | Download protocol |
IHC protocol for ECM1 antibody 68472-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |