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验证数据展示

  • WB analysis using 68472-1-Ig

    WB analysis using 68472-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of human plasma using 68472-1-Ig

    WB analysis of human plasma using 68472-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of human placenta using 68472-1-Ig

    WB analysis of human placenta using 68472-1-Ig

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of A431 using 68472-1-Ig

    WB analysis of A431 using 68472-1-Ig

    A431 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 68472-1-Ig

    WB analysis of K-562 using 68472-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IHC staining of human skin cancer using 68472-1-Ig

    IHC staining of human skin cancer using 68472-1-Ig

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68472-1-Ig (ECM1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with ECM1 antibody included

ECM1 Monoclonal antibody

ECM1 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 68472-1-Ig

产品说明书

CloneNo. 2D1A3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, ELISA

别名

Secretory component p85, extracellular matrix protein 1, 2D1A3

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inhTERT-RPE1 cells, A431 cells, K-562 cells, human placenta tissue, human plasma, THP-1 cells
Positive IHC detected inhuman skin cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy ECM1 Ready-To-Use IHC Kit. ECM1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:2000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

68472-1-Ig targets ECM1 in WB, IHC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 extracellular matrix protein 1
别名 Secretory component p85, extracellular matrix protein 1, 2D1A3
计算分子量 540 aa, 61 kDa
观测分子量60-80 kDa
GenBank蛋白编号BC023505
基因名称 ECM1
Gene ID (NCBI) 1893
RRIDAB_3085183
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16610
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.

实验方案

Product Specific Protocols
WB protocol for ECM1 antibody 68472-1-IgDownload protocol
IHC protocol for ECM1 antibody 68472-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols