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ECM1 Monoclonal antibody, PBS Only (Capture)

ECM1 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Sandwich ELISA, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2D1A3

Cat no : 68472-1-PBS

Print datasheet

Synonyms

Secretory component p85, extracellular matrix protein 1, 2D1A3

验证数据展示

  • WB analysis using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis using 68472-1-Ig (same clone as 68472-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • WB analysis of human plasma using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis of human plasma using 68472-1-Ig (same clone as 68472-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis of human placenta using 68472-1-Ig (same clone as 68472-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis of A431 using 68472-1-Ig (same clone as 68472-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis of K-562 using 68472-1-Ig (same clone as 68472-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50074-1

    Sandwich ELISA standard curve of MP50074-1

    Sandwich ELISA standard curve of MP50074-1, ECM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68472-1-PBS. Detection antibody: 66023-1-PBS. Standard: Eg0533. Range: 0.156-10 ng/mL.

  • IHC staining of human skin cancer using 68472-1-Ig (same clone as 68472-1-PBS)

    IHC staining of human skin cancer using 68472-1-Ig (same clone as 68472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68472-1-Ig (ECM1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECM1 antibody included
Planning an IHC experiment? We recommend our IHCeasy ECM1 Ready-To-Use IHC Kit. ECM1 primary antibody included.

产品信息

68472-1-PBS targets ECM1 as part of a matched antibody pair:

MP50074-1: 68472-1-PBS capture and 66023-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications WB, IHC, Sandwich ELISA, Indirect ELISA Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name extracellular matrix protein 1
Synonyms Secretory component p85, extracellular matrix protein 1, 2D1A3
Calculated Molecular Weight 540 aa, 61 kDa
Observed Molecular Weight60-80 kDa
GenBank Accession NumberBC023505
Gene Symbol ECM1
Gene ID (NCBI) 1893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDQ16610
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.