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验证数据展示

  • WB analysis using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis using 68472-1-Ig (same clone as 68472-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • WB analysis of human plasma using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis of human plasma using 68472-1-Ig (same clone as 68472-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • WB analysis of human placenta using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis of human placenta using 68472-1-Ig (same clone as 68472-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • WB analysis of A431 using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis of A431 using 68472-1-Ig (same clone as 68472-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • WB analysis of K-562 using 68472-1-Ig (same clone as 68472-1-PBS)

    WB analysis of K-562 using 68472-1-Ig (same clone as 68472-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 68472-1-Ig (ECM1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

  • Sandwich ELISA standard curve of MP50074-1

    Sandwich ELISA standard curve of MP50074-1

    Sandwich ELISA standard curve of MP50074-1, ECM1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 68472-1-PBS. Detection antibody: 66023-1-PBS. Standard: Eg0533. Range: 0.156-10 ng/mL.

  • IHC staining of human skin cancer using 68472-1-Ig (same clone as 68472-1-PBS)

    IHC staining of human skin cancer using 68472-1-Ig (same clone as 68472-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 68472-1-Ig (ECM1 antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 68472-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with ECM1 antibody included

ECM1 Monoclonal antibody, PBS Only (Capture)

ECM1 Monoclonal Antibody for WB, IHC, Sandwich ELISA, Indirect ELISA
Cat No. 68472-1-PBS

产品说明书

CloneNo. 2D1A3

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

WB, IHC, Sandwich ELISA, Indirect ELISA

别名

Secretory component p85, extracellular matrix protein 1, 2D1A3

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy ECM1 Ready-To-Use IHC Kit. ECM1 primary antibody included.

产品信息

68472-1-PBS targets ECM1 as part of a matched antibody pair:

MP50074-1: 68472-1-PBS capture and 66023-1-PBS detection (validated in Sandwich ELISA)

Unconjugated mouse monoclonal antibody pair in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IHC, Sandwich ELISA, Indirect ELISA Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 extracellular matrix protein 1
别名 Secretory component p85, extracellular matrix protein 1, 2D1A3
计算分子量 540 aa, 61 kDa
观测分子量60-80 kDa
GenBank蛋白编号BC023505
基因名称 ECM1
Gene ID (NCBI) 1893
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDQ16610
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

ECM1 (extracellular matrix protein 1), also known as URBWD. It is located in secreted, extracellular space, and extracellular matrix, which is mainly expressed in esophagus and gall bladder. The gene encodes a soluble protein that is involved in endochondral bone formation, angiogenesis, and tumor biology. It also interacts with a variety of extracellular and structural proteins, contributing to the maintenance of skin integrity and homeostasis. Mutations in this gene are associated with lipoid proteinosis disorder (also known as hyalinosis cutis et mucosae or Urbach-Wiethe disease) that is characterized by generalized thickening of skin, mucosae and certain viscera. The calculated molecular weight of ECM1 is 60 kDa, and this antibody can recognize the 63 kDa isoform of target, moreover, this protein may have glycosylation modification.