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ECM1 Recombinant antibody, PBS Only (Capture/Detector)

ECM1 Uni-rAbTM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

230321A4

Cat no : 83067-1-PBS

Print datasheet

Synonyms

Secretory component p85, extracellular matrix protein 1, 230321A4

验证数据展示

  • Sandwich ELISA standard curve of MP00011-4

    Sandwich ELISA standard curve of MP00011-4

    Sandwich ELISA standard curve of MP00011-4, Human ECM1 Recombinant Matched Antibody Pair - PBS only. 83067-7-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0533. 83067-1-PBS was HRP conjugated as the detection antibody. Range: 15.6-1000 pg/mL.

  • Sample test of MP00011-4

    Sample test of MP00011-4

    Plasma of fourteen individual healthy human donors was measured. The ECM1 concentration of detected samples was determined to be 3,683.47 ng/mL with a range of 1,495.22-6,198.62 ng/mL.

  • Sample test of MP00011-4

    Sample test of MP00011-4

    MDA-MB-468 were cultured in DMEM  supplemented with 10% fetal calf serum, 50 μM β-mercaptoethanol, 2 mM L-glutamine, 100 U/mL penicillin and 100 μg/mL streptomycin sulfate. The cell culture supernatant was removed, assayed for human ECM1, and measured 11.6 ng/mL.

  • Sample test of MP00011-4

    Sample test of MP00011-4

    Serum of fourteen individual healthy human donors was measured. The ECM1 concentration of detected samples was determined to be 3,875.31 ng/mL with a range of 1,605.39-6,867.83 ng/mL.

  • Cytometric bead array standard curve of MP00011-3

    Cytometric bead array standard curve of MP00011-3

    Cytometric bead array standard curve of MP00011-3, ECM1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83067-1-PBS. Detection antibody: 83067-4-PBS. Standard: Eg0533. Range: 0.625-20 ng/mL.

Planning an IHC experiment? We recommend our IHCeasy ECM1 Ready-To-Use IHC Kit. ECM1 primary antibody included.

产品信息

83067-1-PBS targets ECM1 as part of a matched antibody pair:

MP00011-3: 83067-1-PBS capture and 83067-4-PBS detection (validated in Cytometric bead array)

MP00011-4: 83067-7-PBS capture and 83067-1-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Applications Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test Application Description
Tested Reactivity human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name extracellular matrix protein 1
Synonyms Secretory component p85, extracellular matrix protein 1, 230321A4
Calculated Molecular Weight 540 aa, 61 kDa
GenBank Accession NumberBC023505
Gene Symbol ECM1
Gene ID (NCBI) 1893
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ16610
Storage Buffer 100% PBS pH 7.3
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C