• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 81245-1-RR (same clone as 81245-1-PBS)

    WB analysis using 81245-1-RR (same clone as 81245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81245-1-RR (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81245-1-RR (same clone as 81245-1-PBS)

    WB analysis of HepG2 using 81245-1-RR (same clone as 81245-1-PBS)

    WB result of EEA1 antibody (81245-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81245-1-RR

    Affinity and Kinetic Characterization of 81245-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81245-1-RR against Human EEA1 were performed. The affinity constant is 0.227 nM.

  • Featured Product
  • KD/KO验证

EEA1 Recombinant antibody, PBS Only

EEA1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 81245-1-PBS

产品说明书

CloneNo. 2C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, Indirect ELISA

别名

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

产品信息

81245-1-PBS targets EEA1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EEA1 fusion protein Ag28814 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 early endosome antigen 1
别名 ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1
计算分子量 162 kDa
观测分子量 162 kDa
GenBank蛋白编号NM_003566
基因名称 EEA1
Gene ID (NCBI) 8411
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15075
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.