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  • Featured Product
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EEA1 Recombinant antibody, PBS Only

EEA1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C11

Cat no : 81245-1-PBS

Print datasheet

Synonyms

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

验证数据展示

  • WB analysis using 81245-1-RR (same clone as 81245-1-PBS)

    WB analysis using 81245-1-RR (same clone as 81245-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81245-1-RR (EEA1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 81245-1-RR (same clone as 81245-1-PBS)

    WB analysis of HepG2 using 81245-1-RR (same clone as 81245-1-PBS)

    WB result of EEA1 antibody (81245-1-RR; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEA1 transfected HepG2 cells. This data was developed using the same antibody clone with 81245-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81245-1-RR

    Affinity and Kinetic Characterization of 81245-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81245-1-RR against Human EEA1 were performed. The affinity constant is 0.227 nM.

产品信息

81245-1-PBS targets EEA1 in WB, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat
Immunogen EEA1 fusion protein Ag28814 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name early endosome antigen 1
Synonyms ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberNM_003566
Gene Symbol EEA1
Gene ID (NCBI) 8411
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15075
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.