验证数据展示
经过测试的应用
Positive WB detected in | A431 cells, HepG2 cells, HeLa cells, LNCaP cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
推荐稀释比
应用 | 推荐稀释比 |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81245-1-RR targets EEA1 in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
经测试应用 | WB, ELISA Application Description |
经测试反应性 | Human, Mouse, Rat |
免疫原 | EEA1 fusion protein Ag28814 种属同源性预测 |
宿主/亚型 | Rabbit / IgG |
抗体类别 | Recombinant |
产品类型 | Antibody |
全称 | early endosome antigen 1 |
别名 | ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1 |
计算分子量 | 162 kDa |
观测分子量 | 162 kDa |
GenBank蛋白编号 | NM_003566 |
基因名称 | EEA1 |
Gene ID (NCBI) | 8411 |
RRID | AB_2923711 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | Q15075 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.
实验方案
Product Specific Protocols | |
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WB protocol for EEA1 antibody 81245-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |