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CoraLite® Plus 750-conjugated EEA1 Recombinant antibody

EEA1 Uni-rAbTM Recombinant Antibody for WB
Cat No. CL750-81245

产品说明书

CloneNo. 2C11

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 750
规格: 

-/ -


经过测试的应用

Positive WB detected inHSC-T6 cells, HeLa cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 81245-1-PBS

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-81245 targets EEA1 in WB applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB Application Description
经测试反应性 human, mouse, rat
免疫原 EEA1 fusion protein Ag28814 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 early endosome antigen 1
别名 ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1
计算分子量 162 kDa
观测分子量 162 kDa
GenBank蛋白编号NM_003566
基因名称 EEA1
Gene ID (NCBI) 8411
RRIDAB_3673727
偶联类型 CoraLite® Plus 750 Fluorescent Dye
最大激发/发射波长755 nm / 780 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ15075
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 EEA1 antibody CL750-81245Download protocol
Standard Protocols
Click here to view our Standard Protocols
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