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CoraLite® Plus 750-conjugated EEA1 Recombinant antibody

EEA1 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2C11

Cat No : CL750-81245

Print datasheet

Synonyms

ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1

验证数据展示

  • WB analysis using CL750-81245

    WB analysis using CL750-81245

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-81245 (EEA1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

经过测试的应用

Positive WB detected inHSC-T6 cells, HeLa cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 81245-1-PBS

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL750-81245 targets EEA1 in WB applications and shows reactivity with human, mouse, rat samples.

Tested Applications WB Application Description
Tested Reactivity human, mouse, rat
Immunogen EEA1 fusion protein Ag28814 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name early endosome antigen 1
Synonyms ZFYVE2, MSTP105, MST105, Endosome-associated protein p162, Early endosome antigen 1
Calculated Molecular Weight 162 kDa
Observed Molecular Weight 162 kDa
GenBank Accession NumberNM_003566
Gene Symbol EEA1
Gene ID (NCBI) 8411
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission Maxima Wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ15075
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Early endosome antigen 1 (EEA1) is a marker of early endosomes and has an important role in endosomal trafficking. EEA1 contains coiled-coil regions and an FYVE-type zinc finger which interacts with phosphatidylinositol-3-phosphate. EEA1 functions as a Rab5 effector, mediates endosome docking and, together with SNAREs, leads to membrane fusion.

实验方案

Product Specific Protocols
WB protocol for CL Plus 750 EEA1 antibody CL750-81245Download protocol
Standard Protocols
Click here to view our Standard Protocols