• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

EEF1A1 Monoclonal antibody, PBS Only

EEF1A1 Monoclonal Antibody for WB,Indirect ELISA,IHC,IF,FC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB,Indirect ELISA,IHC,IF,FC

Conjugate

Unconjugated

CloneNo.

1A9F5

Cat No : 67495-1-PBS

Print datasheet

Synonyms

CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, FLJ25721, GRAF 1EF, HNGC:16303, LENG7, PTI1

验证数据展示

  • WB analysis using 67495-1-Ig (same clone as 67495-1-PBS)

    WB analysis using 67495-1-Ig (same clone as 67495-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67495-1-Ig (EEF1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • WB analysis of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

    WB analysis of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

    WB result of EEF1A1 antibody (67495-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1A1 transfected HepG2 cells. This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • IHC staining of human pancreas cancer using 67495-1-Ig (same clone as 67495-1-PBS)

    IHC staining of human pancreas cancer using 67495-1-Ig (same clone as 67495-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 67495-1-Ig (EEF1A1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1A1 antibody included
  • IF Staining of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

    IF Staining of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 67495-1-Ig (EEF1A1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

    FC experiment of HepG2 using 67495-1-Ig (same clone as 67495-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human EEF1A1 (67495-1-Ig, Clone:1A9F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 67495-1-PBS in a different storage buffer formulation.

Planning an IHC experiment? We recommend our IHCeasy EEF1A1 Ready-To-Use IHC Kit. EEF1A1 primary antibody included.

产品信息

67495-1-PBS targets EEF1A1 in WB, IF, FC, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Applications WB,Indirect ELISA,IHC,IF,FC Application Description
Tested Reactivity Human, mouse, rat
Immunogen EEF1A1 fusion protein Ag1938 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation elongation factor 1 alpha 1
Synonyms CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, FLJ25721, GRAF 1EF, HNGC:16303, LENG7, PTI1
Calculated Molecular Weight 462 aa, 50 kDa
Observed Molecular Weight49 kDa
GenBank Accession NumberBC014224
Gene Symbol EEF1A1
Gene ID (NCBI) 1915
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP68104
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

背景介绍

EEF1A1, also named as EEF1A, EF1A and LENG7, belongs to the GTP-binding elongation factor family. This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis. It is a typical housekeeping gene product required for the maintenance of cell proliferation and/or survival. In addition, EEF1A1 protects the aminoester bond against hydrolysis until a correct match between the codon on mRNA and the anti-codon on tRNA can be achieved.