EEF1A1 Recombinant antibody
EEF1A1 Recombinant Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human, Mouse, Rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
CloneNo.
2B16
验证数据展示
经过测试的应用
Positive WB detected in | HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, NIH/3T3 cells, HSC-T6 cells |
Positive IP detected in | HeLa cells |
Positive IHC detected in | mouse brain tissue, human pancreas cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
Immunofluorescence (IF) | IF : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
81377-1-RR targets EEF1A1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Tested Reactivity | Human, Mouse, Rat |
Immunogen | EEF1A1 fusion protein Ag1938 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Full Name | eukaryotic translation elongation factor 1 alpha 1 |
Synonyms | CCS 3, CCS3, EEF 1, EEF1A, eEF1A 1, EEF1A1, EF 1 alpha 1, EF Tu, EF1A, Elongation factor 1 alpha 1, Elongation factor Tu, FLJ25721, GRAF 1EF, HNGC:16303, LENG7, PTI1 |
Calculated Molecular Weight | 462 aa, 50 kDa |
Observed Molecular Weight | 50 kDa |
GenBank Accession Number | BC014224 |
Gene Symbol | EEF1A1 |
Gene ID (NCBI) | 1915 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | P68104 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for EEF1A1 antibody 81377-1-RR | Download protocol |
IHC protocol for EEF1A1 antibody 81377-1-RR | Download protocol |
IF protocol for EEF1A1 antibody 81377-1-RR | Download protocol |
IP protocol for EEF1A1 antibody 81377-1-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |