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验证数据展示

  • WB analysis using 10095-2-AP (same clone as 10095-2-PBS)

    WB analysis using 10095-2-AP (same clone as 10095-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

  • WB analysis of PC-3 using 10095-2-AP (same clone as 10095-2-PBS)

    WB analysis of PC-3 using 10095-2-AP (same clone as 10095-2-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 10095-2-AP (same clone as 10095-2-PBS)

    WB analysis of HeLa using 10095-2-AP (same clone as 10095-2-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10095-2-AP (EEF1B2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 10095-2-AP (same clone as 10095-2-PBS)

    WB analysis of HeLa using 10095-2-AP (same clone as 10095-2-PBS)

    WB result of EEF1B2 antibody (10095-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EEF1B2 transfected HeLa cells. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

  • IP experiment of Jurkat using 10095-2-AP (same clone as 10095-2-PBS)

    IP experiment of Jurkat using 10095-2-AP (same clone as 10095-2-PBS)

    IP result of anti-EEF1B2 (IP:10095-2-AP, 4ug; Detection:10095-2-AP 1:6000) with Jurkat cells lysate 1400 ug. This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 10095-2-AP (same clone as 10095-2-PBS)

    IHC staining of human colon using 10095-2-AP (same clone as 10095-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1B2 antibody included
  • IHC staining of human colon using 10095-2-AP (same clone as 10095-2-PBS)

    IHC staining of human colon using 10095-2-AP (same clone as 10095-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10095-2-AP (EEF1B2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF1B2 antibody included
  • IF Staining of MCF-7 using 10095-2-AP (same clone as 10095-2-PBS)

    IF Staining of MCF-7 using 10095-2-AP (same clone as 10095-2-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using EEF1B2 antibody (10095-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 10095-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO验证

EEF1B2 Polyclonal antibody, PBS Only

EEF1B2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 10095-2-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, IP, Indirect ELISA

别名

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EEF1B2 Ready-To-Use IHC Kit. EEF1B2 primary antibody included.

产品信息

10095-2-PBS targets EEF1B2 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原 EEF1B2 fusion protein Ag0135 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 1 beta 2
别名
计算分子量 25 kDa
观测分子量 30-34 kDa
GenBank蛋白编号BC000211
基因名称 EEF1B2
Gene ID (NCBI) 1933
RRIDAB_2096987
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP24534
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

In eukaryotes, the translation elongation factor eEF1A responsible for transporting amino-acylated tRNA to the ribosome forms a higher-order complex, eEF1H, with its guanine-nucleotide-exchange factor eEF1B. eEF1B consists of three subunits: eEF1B alpha, eEF1B beta and eEF1B gamma. The eEF1B2 possess the nucleotide-exchange activity. Although several models on the basis of in vitro experiments have been proposed for the macromolecular organization of the eEF1H complex, these models differ in various aspects. The human eukaryote elongation factor 1 beta 2 (eEF1B2) migrated as a 30-34 kDa protein in SDS-PAGE. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human EEF1B2.