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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

    IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

    IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (67550-1-Ig, Clone: 1E4D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

EEF2 Monoclonal antibody, PBS Only

EEF2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67550-1-PBS
CloneNo. 1E4D5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.

产品信息

67550-1-PBS targets EEF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, Indirect ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen EEF2 fusion protein Ag13859 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation elongation factor 2
Synonyms Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-
Calculated Molecular Weight 858 aa, 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC126259
Gene Symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_2882765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13639
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.