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Species Reactivity
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Conjugate
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验证数据展示

  • WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

    IHC staining of human breast cancer using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with EEF2 antibody included
  • WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    WB analysis using 67550-1-Ig (same clone as 67550-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

    IF Staining of HepG2 using 67550-1-Ig (same clone as 67550-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (67550-1-Ig, Clone: 1E4D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67550-1-PBS in a different storage buffer formulation.

EEF2 Monoclonal antibody, PBS Only

EEF2 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67550-1-PBS

产品说明书

CloneNo. 1E4D5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.

产品信息

67550-1-PBS targets EEF2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat, pig
免疫原 EEF2 fusion protein Ag13859 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 2
别名 Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-
计算分子量 858 aa, 95 kDa
观测分子量 100 kDa
GenBank蛋白编号BC126259
基因名称 EEF2
Gene ID (NCBI) 1938
RRIDAB_2882765
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13639
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

EEF2 (Eukaryotic Elongation Factor 2), also known as EF-2 and EF2, is a crucial protein involved in the process of protein synthesis in eukaryotic cells. It plays a pivotal role in the elongation phase of translation, where it facilitates the movement of the ribosome along the mRNA strand. This movement is essential for the addition of amino acids to the growing polypeptide chain.