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验证数据展示

  • WB analysis using 67550-1-Ig

    WB analysis using 67550-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67550-1-Ig

    IHC staining of human breast cancer using 67550-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67550-1-Ig (EEF2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EEF2 antibody included
  • WB analysis using 67550-1-Ig

    WB analysis using 67550-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67550-1-Ig (EEF2 antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67550-1-Ig

    IF Staining of HepG2 using 67550-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using EEF2 antibody (67550-1-Ig, Clone: 1E4D5 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67550-1-Ig
CloneNo. 1E4D5

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHeLa cells, HSC-T6 cells, LoVo cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, 4T1 cells, MCF-7 cells, pig brain tissue
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488, CoraLite®555 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67550-1-Ig targets EEF2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Applications WB, IHC, IF/ICC, ELISA Application Description
Tested Reactivity human, mouse, rat, pig
Immunogen EEF2 fusion protein Ag13859 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation elongation factor 2
Synonyms Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-
Calculated Molecular Weight 858 aa, 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC126259
Gene Symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_2882765
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13639
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
WB protocol for EEF2 antibody 67550-1-IgDownload protocol
IHC protocol for EEF2 antibody 67550-1-IgDownload protocol
IF protocol for EEF2 antibody 67550-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols