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CoraLite®555-conjugated EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

1E4D5

Cat no : CL555-67550

Print datasheet

Synonyms

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

验证数据展示

  • IF Staining of HepG2 using CL555-67550

    IF Staining of HepG2 using CL555-67550

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 EEF2 antibody (CL555-67550, Clone: 1E4D5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-67550

    FC experiment of HeLa using CL555-67550

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human EEF2 (CL555-67550, Clone:1E4D5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67550-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67550 targets EEF2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications IF/ICC, FC (Intra) Application Description
Tested Reactivity human, mouse, rat
Immunogen EEF2 fusion protein Ag13859 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation elongation factor 2
Synonyms Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-
Calculated Molecular Weight 858 aa, 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC126259
Gene Symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_2919703
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission Maxima Wavelengths557 nm / 570 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13639
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL555 EEF2 antibody CL555-67550Download protocol
FC protocol for CL555 EEF2 antibody CL555-67550Download protocol
Standard Protocols
Click here to view our Standard Protocols