• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of HepG2 using CL555-67550

    IF Staining of HepG2 using CL555-67550

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®555 EEF2 antibody (CL555-67550, Clone: 1E4D5 ) at dilution of 1:200.

  • FC experiment of HeLa using CL555-67550

    FC experiment of HeLa using CL555-67550

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®555 Anti-Human EEF2 (CL555-67550, Clone:1E4D5) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

CoraLite®555-conjugated EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL555-67550

产品说明书

CloneNo. 1E4D5

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®555
规格: 

-/ -

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67550-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL555-67550 targets EEF2 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 EEF2 fusion protein Ag13859 种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation elongation factor 2
别名 Elongation factor 2, EF-2, EF2, EF 2, EC:3.6.5.-
计算分子量 858 aa, 95 kDa
观测分子量 100 kDa
GenBank蛋白编号BC126259
基因名称 EEF2
Gene ID (NCBI) 1938
RRIDAB_2919703
偶联类型 CoraLite®555 Fluorescent Dye
最大激发/发射波长557 nm / 570 nm
形式 Liquid
纯化方式Protein G purification
UNIPROT IDP13639
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL555 EEF2 antibody CL555-67550Download protocol
FC protocol for CL555 EEF2 antibody CL555-67550Download protocol
Standard Protocols
Click here to view our Standard Protocols