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CoraLite® Plus 488-conjugated EEF2 Monoclonal antibody

EEF2 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4D5

Cat no : CL488-67550

Print datasheet

Synonyms

EEF 2, EEF2, EF 2, EF2, Elongation factor 2

验证数据展示

  • IF Staining of HepG2 using CL488-67550

    IF Staining of HepG2 using CL488-67550

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 EEF2 antibody (CL488-67550, Clone: 1E4D5 ) at dilution of 1:200.

经过测试的应用

Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy EEF2 Ready-To-Use IHC Kit. EEF2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67550-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67550 targets EEF2 in IF/ICC applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human, mouse, rat
Immunogen EEF2 fusion protein Ag13859 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name eukaryotic translation elongation factor 2
Synonyms EEF 2, EEF2, EF 2, EF2, Elongation factor 2
Calculated Molecular Weight 858 aa, 95 kDa
Observed Molecular Weight 100 kDa
GenBank Accession NumberBC126259
Gene Symbol EEF2
Gene ID (NCBI) 1938
RRIDAB_2919498
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP13639
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EEF2 antibody CL488-67550Download protocol
Standard Protocols
Click here to view our Standard Protocols