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验证数据展示

  • WB analysis using 31624-1-AP

    WB analysis using 31624-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31624-1-AP (EFHD2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 31624-1-AP

    IHC staining of mouse brain using 31624-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31624-1-AP (EFHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EFHD2 antibody included
  • IHC staining of mouse brain using 31624-1-AP

    IHC staining of mouse brain using 31624-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31624-1-AP (EFHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EFHD2 antibody included

EFHD2 Polyclonal antibody

EFHD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31624-1-AP

Print datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, NCI-H1299 cells, SW480 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EFHD2 Ready-To-Use IHC Kit. EFHD2 primary antibody included.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31624-1-AP targets EFHD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Applications WB, IHC, ELISA Application Description
Tested Reactivity human, mouse
Immunogen EFHD2 fusion protein Ag35084 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name EF-hand domain family, member D2
Synonyms SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2
Calculated Molecular Weight27 kDa
Observed Molecular Weight27-30 kDa
GenBank Accession NumberBC023611
Gene Symbol EFHD2
Gene ID (NCBI) 79180
RRIDAB_3670053
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96C19
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EF-hand domain-containing protein D2 (also known as Swiprosin-1) is a calcium-binding protein with two conserved EF-chiral coiled-coil structural domains, and it is mostly involved in calcium signaling, cytoskeleton assembly and synapse formation. EFHD2 is broadly expressed in various cell types, with particularly high expression in neurons.

实验方案

Product Specific Protocols
WB protocol for EFHD2 antibody 31624-1-APDownload protocol
IHC protocol for EFHD2 antibody 31624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols