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验证数据展示

  • WB analysis using 31624-1-AP

    WB analysis using 31624-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 31624-1-AP (EFHD2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse brain using 31624-1-AP

    IHC staining of mouse brain using 31624-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31624-1-AP (EFHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EFHD2 antibody included
  • IHC staining of mouse brain using 31624-1-AP

    IHC staining of mouse brain using 31624-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 31624-1-AP (EFHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with EFHD2 antibody included

EFHD2 Polyclonal antibody

EFHD2 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 31624-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, ELISA

别名

SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inA549 cells, NCI-H1299 cells, SW480 cells
Positive IHC detected inmouse brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Planning an IHC experiment? We recommend our IHCeasy EFHD2 Ready-To-Use IHC Kit. EFHD2 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

31624-1-AP targets EFHD2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原 EFHD2 fusion protein Ag35084 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 EF-hand domain family, member D2
别名 SWS1, Swiprosin-1, Swiprosin 1, EF-hand domain-containing protein D2
计算分子量27 kDa
观测分子量27-30 kDa
GenBank蛋白编号BC023611
基因名称 EFHD2
Gene ID (NCBI) 79180
RRIDAB_3670053
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity Purification
UNIPROT IDQ96C19
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EF-hand domain-containing protein D2 (also known as Swiprosin-1) is a calcium-binding protein with two conserved EF-chiral coiled-coil structural domains, and it is mostly involved in calcium signaling, cytoskeleton assembly and synapse formation. EFHD2 is broadly expressed in various cell types, with particularly high expression in neurons.

实验方案

Product Specific Protocols
WB protocol for EFHD2 antibody 31624-1-APDownload protocol
IHC protocol for EFHD2 antibody 31624-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols