EGFR Polyclonal antibody
EGFR Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
Human
Applications
WB, IF/ICC, IP, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | A431 cells |
Positive IP detected in | HeLa cells |
Positive IF/ICC detected in | A431 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:750-1:3000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
30139-1-AP targets EGFR in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human samples.
Tested Applications | WB, IF/ICC, IP, ELISA Application Description |
Tested Reactivity | Human |
Immunogen | fusion protein 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) |
Synonyms | EGFR, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1 |
Calculated Molecular Weight | 134kd |
Observed Molecular Weight | 145-165 kDa |
GenBank Accession Number | NM_005228.5 |
Gene Symbol | EGFR |
Gene ID (NCBI) | 1956 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | P00533 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for EGFR antibody 30139-1-AP | Download protocol |
IF protocol for EGFR antibody 30139-1-AP | Download protocol |
IP protocol for EGFR antibody 30139-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |