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验证数据展示

  • WB analysis using 30139-1-AP

    WB analysis using 30139-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 30139-1-AP (EGFR antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • IF Staining of A431 using 30139-1-AP

    IF Staining of A431 using 30139-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using EGFR antibody (30139-1-AP) at dilution of 1:1500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • IP experiment of HeLa using 30139-1-AP

    IP experiment of HeLa using 30139-1-AP

    IP result of anti-EGFR (IP:30139-1-AP, 4ug; Detection:30139-1-AP 1:4000) with HeLa cells lysate 1520 ug.

  • IF Staining of HaCaT using 30139-1-AP

    IF Staining of HaCaT using 30139-1-AP

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using EGFR antibody (30139-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • FC experiment of A431 using 30139-1-AP

    FC experiment of A431 using 30139-1-AP

    1x10^6 A431 cells were surface stained with 0.25 ug EGFR Polyclonal antibody (30139-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were not fixed.

EGFR Polyclonal antibody

EGFR Polyclonal Antibody for WB, IF/ICC, FC, IP, ELISA
Cat No. 30139-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IF/ICC, FC, IP, ELISA

别名

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive WB detected inA431 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inA431 cells, HaCaT cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:750-1:3000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

30139-1-AP targets EGFR in WB, IF/ICC, FC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, FC, IP, ELISA Application Description
经测试反应性 human
免疫原 fusion protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R
计算分子量134kd
观测分子量 145-165 kDa
GenBank蛋白编号NM_005228.5
基因名称 EGFR
Gene ID (NCBI) 1956
RRIDAB_3086243
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00533
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer.

实验方案

Product Specific Protocols
WB protocol for EGFR antibody 30139-1-APDownload protocol
IF protocol for EGFR antibody 30139-1-APDownload protocol
IP protocol for EGFR antibody 30139-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols