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CoraLite® Plus 488-conjugated EGFR Polyclonal antibody

EGFR Polyclonal Antibody for IF/ICC, FC

Host / Isotype

Rabbit / IgG

Reactivity

Human

Applications

IF/ICC, FC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat No : CL488-30139

Print datasheet

Synonyms

EGFR, EGFRviii, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1

验证数据展示

  • IF Staining of A431 using CL488-30139

    IF Staining of A431 using CL488-30139

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 EGFR antibody (CL488-30139) at dilution of 1:200.

  • FC experiment of A431 using CL488-30139

    FC experiment of A431 using CL488-30139

    1x10^6 A431 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 EGFR Polyclonal Antibody (CL488-30139)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were not fixed.

经过测试的应用

Positive IF detected inA431 cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 30139-1-AP

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-30139 targets EGFR in IF, FC applications and shows reactivity with Human samples.

Tested Applications IF/ICC, FC
Tested Reactivity Human
Immunogen Recombinant protein 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
Synonyms EGFR, EGFRviii, ERBB, ERBB1, HER1, mENA, PIG61, Proto oncogene c ErbB 1
Calculated Molecular Weight134kd
Observed Molecular Weight 145-165 kDa
GenBank Accession NumberNM_005228.5
Gene Symbol EGFR
Gene ID (NCBI) 1956
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDP00533
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EGFR antibody CL488-30139Download protocol
FC protocol for CL Plus 488 EGFR antibody CL488-30139Download protocol
Standard Protocols
Click here to view our Standard Protocols