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验证数据展示

  • IF Staining of A431 using CL488-30139

    IF Staining of A431 using CL488-30139

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite® Plus 488 EGFR antibody (CL488-30139) at dilution of 1:200.

  • IF Staining of HaCaT using CL488-30139

    IF Staining of HaCaT using CL488-30139

    Immunofluorescent analysis of (-20°C Methanol) fixed HaCaT cells using CoraLite® Plus 488 EGFR antibody (CL488-30139) at dilution of 1:200.

  • FC experiment of A431 using CL488-30139

    FC experiment of A431 using CL488-30139

    1x10^6 A431 cells were surface stained with 0.4 ug Coralite® Plus 488 EGFR Polyclonal Antibody (CL488-30139)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were not fixed.

CoraLite® Plus 488-conjugated EGFR Polyclonal antibody

EGFR Polyclonal Antibody for IF/ICC, FC
Cat No. CL488-30139

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IF/ICC, FC

别名

Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 488
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inA431 cells, HaCaT cells
Positive FC detected inA431 cells
Planning an IHC experiment? We recommend our IHCeasy EGFR Ready-To-Use IHC Kit. EGFR primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 30139-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-30139 targets EGFR in IF/ICC, FC applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC Application Description
经测试反应性 human
免疫原 Recombinant protein 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian)
别名 Proto-oncogene c-ErbB-1, Epidermal growth factor receptor, EGF Receptor , EGF R
计算分子量134kd
观测分子量 145-165 kDa
GenBank蛋白编号NM_005228.5
基因名称 EGFR
Gene ID (NCBI) 1956
RRIDAB_3672842
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP00533
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

EGFR is a receptor for EGF, but also for other members of the EGF family, as TGF-alpha, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. ERBB1 is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with C-SRC and CTNNB1/beta-catenin. Isoform 2/truncated isoform of ERBB1 may act as an antagonist. EGFR is associated with lung cancer.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 EGFR antibody CL488-30139Download protocol
FC protocol for CL Plus 488 EGFR antibody CL488-30139Download protocol
Standard Protocols
Click here to view our Standard Protocols