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验证数据展示

  • WB analysis of HEK-293 using 67601-1-Ig

    WB analysis of HEK-293 using 67601-1-Ig

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 67601-1-Ig (EIF1 antibody) at dilution of 1:3200 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 67601-1-Ig

    IF Staining of HepG2 using 67601-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67601-1-Ig (EIF1 antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

EIF1 Monoclonal antibody

EIF1 Monoclonal Antibody for WB, IF/ICC, ELISA
Cat No. 67601-1-Ig

Print datasheet

CloneNo. 2G8C6

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IF/ICC, ELISA

Synonyms

A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size: 

-/ -

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells
Positive IF/ICC detected inHepG2 cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6400
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67601-1-Ig targets EIF1 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.

Tested Applications WB, IF/ICC, ELISA Application Description
Tested Reactivity Human
Immunogen EIF1 fusion protein Ag7843 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 1
Synonyms A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC005118
Gene Symbol EIF1
Gene ID (NCBI) 10209
RRIDAB_2882807
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41567
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd.

实验方案

Product Specific Protocols
WB protocol for EIF1 antibody 67601-1-IgDownload protocol
IF protocol for EIF1 antibody 67601-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols