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验证数据展示

  • IF Staining of HepG2 using CL594-67601

    IF Staining of HepG2 using CL594-67601

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 EIF1 antibody (CL594-67601, Clone: 2G8C6 ) at dilution of 1:200.

  • IF Staining of MCF-7 using CL594-67601

    IF Staining of MCF-7 using CL594-67601

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 EIF1 antibody (CL594-67601, Clone: 2G8C6 ) at dilution of 1:200.

CoraLite®594-conjugated EIF1 Monoclonal antibody

EIF1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67601

Print datasheet

CloneNo. 2G8C6

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Synonyms

A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite®594
Size: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67601-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67601 targets EIF1 in IF/ICC applications and shows reactivity with Human samples.

Tested Applications IF/ICC Application Description
Tested Reactivity Human
Immunogen EIF1 fusion protein Ag7843 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name eukaryotic translation initiation factor 1
Synonyms A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1
Calculated Molecular Weight 13 kDa
Observed Molecular Weight 13 kDa
GenBank Accession NumberBC005118
Gene Symbol EIF1
Gene ID (NCBI) 10209
RRIDAB_2920163
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP41567
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd.

实验方案

Product Specific Protocols
IF protocol for CL594 EIF1 antibody CL594-67601Download protocol
Standard Protocols
Click here to view our Standard Protocols