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验证数据展示

  • IF Staining of HepG2 using CL594-67601

    IF Staining of HepG2 using CL594-67601

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite®594 EIF1 antibody (CL594-67601, Clone: 2G8C6 ) at dilution of 1:200.

  • IF Staining of MCF-7 using CL594-67601

    IF Staining of MCF-7 using CL594-67601

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using CoraLite®594 EIF1 antibody (CL594-67601, Clone: 2G8C6 ) at dilution of 1:200.

CoraLite®594-conjugated EIF1 Monoclonal antibody

EIF1 Monoclonal Antibody for IF/ICC
Cat No. CL594-67601

产品说明书

CloneNo. 2G8C6

宿主/亚型

Mouse / IgG2b

种属反应性

Human

应用

IF/ICC

别名

A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite®594
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inMCF-7 cells, HepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67601-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67601 targets EIF1 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原 EIF1 fusion protein Ag7843 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 eukaryotic translation initiation factor 1
别名 A121, EIF 1, EIF1, EIF1A, ISO1, SUI1, Sui1iso1
计算分子量 13 kDa
观测分子量 13 kDa
GenBank蛋白编号BC005118
基因名称 EIF1
Gene ID (NCBI) 10209
RRIDAB_2920163
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP41567
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

It is a necessary for scanning and involved in initiation site selection. Promotes the assembly of 48S ribosomal complexes at the authentic initiation codon of a conventional capped mRNA. Molecular weight is 13kd.

实验方案

Product Specific Protocols
IF protocol for CL594 EIF1 antibody CL594-67601Download protocol
Standard Protocols
Click here to view our Standard Protocols